Vascular smooth muscle cell-directed overexpression of heme oxygenase-1 elevates blood pressure through attenuation of nitric oxide-induced vasodilation in mice

Tomihiko Imai, Toshisuke Morita, Takayuki Shindo, Ryozo Nagai, Yoshio Yazaki, Hiroki Kurihara, Makoto Suematsu, Shigehiro Katayama

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

To elucidate pathophysiological roles of heme oxygenase (HO)-1 in regulation of vascular tone in vivo, we have developed and characterized transgenic (Tg) mice that overexpress HO-1 site specifically in vascular smooth muscle cells (VSMCs). The Tg mice were generated by use of human HO-1 cDNA under the control of SM22-α promoter. The HO-1 gene overexpression was demonstrated by Northern blot analysis and coincided with increases in the protein expression in VSMCs and total HO activities. Tg mice exhibited a significant increase in arterial pressure at various ages and displayed impaired nitrovasodilatory responses in isolated aortic segments versus nontransgenic littermates while enhancing their nitric oxide (NO) production. The pressure of Tg mice was unchanged by systemic administration of either Nω-nitro-L-arginine or SNP. Furthermore, the isolated aorta in these mice exhibited lesser extents of NO-elicited cGMP elevation via soluble guanylate cyclase (sGC), while exhibiting no notable downregulation of sGC expression. Such impairment of the NO-elicited cGMP increase was restored significantly by tin protoporphyrin IX, an HO inhibitor. On the other hand, 3-(5′-hydroxymethyl-2′ furyl)-1-benzyl-indazol (YC-1), an NO-independent activator of sGC, increased cGMP and relaxed aortas from Tg mice to levels comparable with those from nontransgenic mice, which indicates that contents of functionally intact sGC are unlikely to differ between the two systems. These findings suggest that site-specific overexpression of HO-1 in VSMCs suppresses vasodilatory response to NO and thereby leads to an elevation of arterial pressure.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalCirculation Research
Volume89
Issue number1
Publication statusPublished - 2001 Jul 6

Fingerprint

Heme Oxygenase-1
Vascular Smooth Muscle
Vasodilation
Transgenic Mice
Smooth Muscle Myocytes
Nitric Oxide
Blood Pressure
Heme Oxygenase (Decyclizing)
Aorta
Arterial Pressure
Northern Blotting
Single Nucleotide Polymorphism
Blood Vessels
Arginine
Down-Regulation
Complementary DNA
Pressure
Soluble Guanylyl Cyclase
Genes
Proteins

Keywords

  • Carbon monoxide
  • Heme oxygenase-1
  • Hypertension
  • Soluble guanylate cyclase
  • Transgenic mice

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Vascular smooth muscle cell-directed overexpression of heme oxygenase-1 elevates blood pressure through attenuation of nitric oxide-induced vasodilation in mice. / Imai, Tomihiko; Morita, Toshisuke; Shindo, Takayuki; Nagai, Ryozo; Yazaki, Yoshio; Kurihara, Hiroki; Suematsu, Makoto; Katayama, Shigehiro.

In: Circulation Research, Vol. 89, No. 1, 06.07.2001, p. 55-62.

Research output: Contribution to journalArticle

Imai, T, Morita, T, Shindo, T, Nagai, R, Yazaki, Y, Kurihara, H, Suematsu, M & Katayama, S 2001, 'Vascular smooth muscle cell-directed overexpression of heme oxygenase-1 elevates blood pressure through attenuation of nitric oxide-induced vasodilation in mice', Circulation Research, vol. 89, no. 1, pp. 55-62.
Imai, Tomihiko ; Morita, Toshisuke ; Shindo, Takayuki ; Nagai, Ryozo ; Yazaki, Yoshio ; Kurihara, Hiroki ; Suematsu, Makoto ; Katayama, Shigehiro. / Vascular smooth muscle cell-directed overexpression of heme oxygenase-1 elevates blood pressure through attenuation of nitric oxide-induced vasodilation in mice. In: Circulation Research. 2001 ; Vol. 89, No. 1. pp. 55-62.
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