Vascular smooth muscle contraction induced by Na+ channel activators, veratridine and batrachotoxin

Masayoshi Shinjo(H), Nakaki Toshio, Otsuka Yukari, Sasakawa Nobuyuki, Kato Ryuichi

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The effects of the sodium channel activators veratridine and batrachotoxin on isolated rat aorta were investigated. Veratridine caused gradual contraction, independent of the presence of endolhelium, with an EC50 of 35 μM. Batrachotoxin (1 μM) also induced contraction. Both effects were completely inhibited by the sodium channel blocker tetrodotoxin (1 μM). The veratridine (60 μM)-induced contraction was inhibited by nifedipine (0.1 μM). In the absence of extracellular Ca2+, veratridine (60 μm) did not cause contraction. Sodium nitroprusside (80 nM), acetylcholine (10 μM) and isoproterenol (1 μM) caused relaxation of rings precontracted with veratridine (60 μM). An inhibitor of endothelium-derived relaxing factor (EDRF) synthase, Nω-nitro-L-arginine methyl ester (L-NAME) (0.65 mM). enhanced the veratridine-induced contraction in rings with an intact endothelium, which suggests that EDRF was being released during the veratridine-induced contraction. These results show that the activation of sodium channels on smooth muscle cells induces a contraction that is probably mediated by Ca2+ influx through voltage-dependent Ca2+ channels.

Original languageEnglish
Pages (from-to)199-202
Number of pages4
JournalEuropean Journal of Pharmacology
Volume205
Issue number2
DOIs
Publication statusPublished - 1991 Nov 26

Fingerprint

Batrachotoxins
Veratridine
Muscle Contraction
Vascular Smooth Muscle
Endothelium-Dependent Relaxing Factors
Sodium Channel Agonists
Sodium Channel Blockers
Sodium Channels
NG-Nitroarginine Methyl Ester
Tetrodotoxin
Nitroprusside
Nifedipine
Isoproterenol
Acetylcholine
Smooth Muscle Myocytes
Endothelium
Aorta

Keywords

  • Batrachotoxin
  • Blood vessels
  • Contraction
  • Na channels
  • Smooth muscle
  • Veratridine

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Vascular smooth muscle contraction induced by Na+ channel activators, veratridine and batrachotoxin. / Shinjo(H), Masayoshi; Toshio, Nakaki; Yukari, Otsuka; Nobuyuki, Sasakawa; Ryuichi, Kato.

In: European Journal of Pharmacology, Vol. 205, No. 2, 26.11.1991, p. 199-202.

Research output: Contribution to journalArticle

Shinjo(H), Masayoshi ; Toshio, Nakaki ; Yukari, Otsuka ; Nobuyuki, Sasakawa ; Ryuichi, Kato. / Vascular smooth muscle contraction induced by Na+ channel activators, veratridine and batrachotoxin. In: European Journal of Pharmacology. 1991 ; Vol. 205, No. 2. pp. 199-202.
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