Vascular tissue fragility assessed by a new double stain method

Masahiko Zuka, Yasunori Okada, Ryoichi Nemori, Akihiro Fukuda, Noboru Takekoshi, Isao Nakanishi, Shogo Katsuda

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Although matrix metalloproteinases (MMPs) are known to be involved in the development of atherosclerosis and the instability of atheromatous plaques, much remains to be learned about their roles at the tissue level. To help clarify this area, we established a new double staining method using film in situ zymography and immunohistochemistry. Using this technique, a comprehensive analysis of the gelatinolytic activity in human vessel tissue demonstrated that gelatinolytic activity is enhanced in the shoulder region and fibrous cap at superficial areas of the atheromatous plaque in the presence of thrombolysis. Enzyme assay clarified high activity in the superficial area (7.50 ± 5.04 U/mg weight; P < 0.001). Gelatin zymography also indicated that addition of the antiplatelet agent, trapidil, alters the amount of secretion of MMP-2 and MMP-9 and their activation ratio. This novel approach to detect the activity of gelatinases in resected tissues may aid in the selection of optimal treatment of individual patients.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalApplied Immunohistochemistry and Molecular Morphology
Issue number1
Publication statusPublished - 2003 Mar
Externally publishedYes


  • Acute coronary syndrome
  • Immunogold-silver staining
  • In situ zymography
  • MMP-9 protein
  • Thrombosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Medical Laboratory Technology


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