Vasodilator response of mesenteric arterioles to histamine in spontaneously hypertensive rats

Hidekazu Suzuki, B. W. Zweifach, G. W. Schmid-Schonbein

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Recent evidence suggests that spontaneously hypertensive rats (SHR) exhibit an impaired response to inflammatory mediators. We designed this study to analyze the response of arterioles of SHR after stimulation with a proinflammatory agent, histamine, compared with the response of arterioles of normotensive Wistar-Kyoto (WKY) controls. We observed mesenteric arterioles by intravital microscopy in rats under general anesthesia and measured their lumen diameters after histamine superfusion. To compare the concentration- response curve with histamine, we also studied the effect of an endothelium- dependent vasodilator, acetylcholine, and an independent vasodilator, sodium nitroprusside. At the end of each experiment we applied papaverine topically to determine the maximal diameter for each vessel, from which we computed arteriolar tone. Arteriolar tone in SHR is set at a higher steady state level than in WKY. The concentration required for a 50% dilator response (EC50) of histamine in SHR was significantly higher than that in WKY. In SHR the arteriolar response showed the same refractory pattern to histamine as to acetylcholine. In contrast, the EC50 of sodium nitroprusside in SHR was similar to that in WKY. Our results indicate that SHR exhibit an impaired dilator response to histamine that is due to a blunted endothelium-dependent vasodilation.

Original languageEnglish
Pages (from-to)397-400
Number of pages4
JournalHypertension
Volume26
Issue number3
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Arterioles
Inbred SHR Rats
Vasodilator Agents
Histamine
Nitroprusside
Acetylcholine
Histamine Agents
Endothelium-Dependent Relaxing Factors
Papaverine
Vasodilation
General Anesthesia
Endothelium

Keywords

  • acetylcholine
  • endothelium
  • histamine
  • microcirculation
  • sodium nitroprusside

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Suzuki, H., Zweifach, B. W., & Schmid-Schonbein, G. W. (1995). Vasodilator response of mesenteric arterioles to histamine in spontaneously hypertensive rats. Hypertension, 26(3), 397-400.

Vasodilator response of mesenteric arterioles to histamine in spontaneously hypertensive rats. / Suzuki, Hidekazu; Zweifach, B. W.; Schmid-Schonbein, G. W.

In: Hypertension, Vol. 26, No. 3, 1995, p. 397-400.

Research output: Contribution to journalArticle

Suzuki, H, Zweifach, BW & Schmid-Schonbein, GW 1995, 'Vasodilator response of mesenteric arterioles to histamine in spontaneously hypertensive rats', Hypertension, vol. 26, no. 3, pp. 397-400.
Suzuki, Hidekazu ; Zweifach, B. W. ; Schmid-Schonbein, G. W. / Vasodilator response of mesenteric arterioles to histamine in spontaneously hypertensive rats. In: Hypertension. 1995 ; Vol. 26, No. 3. pp. 397-400.
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