Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity

Akiyo Natsubori, Iku Tsutsui-Kimura, Hiroshi Nishida, Youcef Bouchekioua, Hiroshi Sekiya, Motokazu Uchigashima, Masahiko Watanabe, Alban De Kerchove D’Exaerde, Masaru Mimura, Norio Takata, Kenji F. Tanaka

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The ventral striatum is involved in motivated behavior. Akin to the dorsal striatum, the ventral striatum contains two parallel pathways: the striatomesencephalic pathway consisting of dopamine receptor Type 1-expressing medium spiny neurons (D1-MSNs) and the striatopallidal pathway consisting of D2-MSNs. These two genetically identified pathways are thought to encode opposing functions in motivated behavior. It has also been reported that D1/D2 genetic selectivity is not attributed to the anatomical discrimination of two pathways. We wanted to determine whether D1-and D2-MSNs in the ventral striatum functioned in an opposing manner as previous observations claimed, and whether D1/D2 selectivity corresponded to a functional segregation in motivated behavior of mice. To address this question, we focused on the lateral portion of ventral striatum as a region implicated in food-incentive, goal-directed behavior, and recorded D1 or D2-MSN activity by using a gene-encoded ratiometric Ca2+ indicator and by constructing a fiberphotometry system, and manipulated their activities via optogenetic inhibition during ongoing behaviors. We observed concurrent event-related compound Ca2+ elevations in ventrolateral D1-and D2-MSNs, especially at trial start cue-related and first lever press-related times. D1 or D2 selective optogenetic inhibition just after the trial start cue resulted in a reduction of goal-directed behavior, indicating a shared coding of motivated behavior by both populations at this time. Only D1-selective inhibition just after the first lever press resulted in the reduction of behavior, indicating D1-MSN-specific coding at that specific time. Our data did not support opposing encoding by both populations in food-incentive, goal-directed behavior.

Original languageEnglish
Pages (from-to)2723-2733
Number of pages11
JournalJournal of Neuroscience
Volume37
Issue number10
DOIs
Publication statusPublished - 2017 Mar 8

Keywords

  • Fiberphotometry
  • Food-incentive goal-directed behavior
  • Motivation
  • Optogenetic inhibition
  • Ventrolateral striatum
  • Yellow cameleon

ASJC Scopus subject areas

  • Neuroscience(all)

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    Natsubori, A., Tsutsui-Kimura, I., Nishida, H., Bouchekioua, Y., Sekiya, H., Uchigashima, M., Watanabe, M., De Kerchove D’Exaerde, A., Mimura, M., Takata, N., & Tanaka, K. F. (2017). Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity. Journal of Neuroscience, 37(10), 2723-2733. https://doi.org/10.1523/JNEUROSCI.3377-16.2017