Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity

Akiyo Natsubori, Iku Tsutsui-Kimura, Hiroshi Nishida, Youcef Bouchekioua, Hiroshi Sekiya, Motokazu Uchigashima, Masahiko Watanabe, Alban De Kerchove D’Exaerde, Masaru Mimura, Norio Takata, Kenji Tanaka

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The ventral striatum is involved in motivated behavior. Akin to the dorsal striatum, the ventral striatum contains two parallel pathways: the striatomesencephalic pathway consisting of dopamine receptor Type 1-expressing medium spiny neurons (D1-MSNs) and the striatopallidal pathway consisting of D2-MSNs. These two genetically identified pathways are thought to encode opposing functions in motivated behavior. It has also been reported that D1/D2 genetic selectivity is not attributed to the anatomical discrimination of two pathways. We wanted to determine whether D1-and D2-MSNs in the ventral striatum functioned in an opposing manner as previous observations claimed, and whether D1/D2 selectivity corresponded to a functional segregation in motivated behavior of mice. To address this question, we focused on the lateral portion of ventral striatum as a region implicated in food-incentive, goal-directed behavior, and recorded D1 or D2-MSN activity by using a gene-encoded ratiometric Ca2+ indicator and by constructing a fiberphotometry system, and manipulated their activities via optogenetic inhibition during ongoing behaviors. We observed concurrent event-related compound Ca2+ elevations in ventrolateral D1-and D2-MSNs, especially at trial start cue-related and first lever press-related times. D1 or D2 selective optogenetic inhibition just after the trial start cue resulted in a reduction of goal-directed behavior, indicating a shared coding of motivated behavior by both populations at this time. Only D1-selective inhibition just after the first lever press resulted in the reduction of behavior, indicating D1-MSN-specific coding at that specific time. Our data did not support opposing encoding by both populations in food-incentive, goal-directed behavior.

Original languageEnglish
Pages (from-to)2723-2733
Number of pages11
JournalJournal of Neuroscience
Volume37
Issue number10
DOIs
Publication statusPublished - 2017 Mar 8

Fingerprint

Corpus Striatum
Motivation
Neurons
Food
Optogenetics
Cues
Dopamine Receptors
Population
Ventral Striatum

Keywords

  • Fiberphotometry
  • Food-incentive goal-directed behavior
  • Motivation
  • Optogenetic inhibition
  • Ventrolateral striatum
  • Yellow cameleon

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity. / Natsubori, Akiyo; Tsutsui-Kimura, Iku; Nishida, Hiroshi; Bouchekioua, Youcef; Sekiya, Hiroshi; Uchigashima, Motokazu; Watanabe, Masahiko; De Kerchove D’Exaerde, Alban; Mimura, Masaru; Takata, Norio; Tanaka, Kenji.

In: Journal of Neuroscience, Vol. 37, No. 10, 08.03.2017, p. 2723-2733.

Research output: Contribution to journalArticle

Natsubori, A, Tsutsui-Kimura, I, Nishida, H, Bouchekioua, Y, Sekiya, H, Uchigashima, M, Watanabe, M, De Kerchove D’Exaerde, A, Mimura, M, Takata, N & Tanaka, K 2017, 'Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity', Journal of Neuroscience, vol. 37, no. 10, pp. 2723-2733. https://doi.org/10.1523/JNEUROSCI.3377-16.2017
Natsubori, Akiyo ; Tsutsui-Kimura, Iku ; Nishida, Hiroshi ; Bouchekioua, Youcef ; Sekiya, Hiroshi ; Uchigashima, Motokazu ; Watanabe, Masahiko ; De Kerchove D’Exaerde, Alban ; Mimura, Masaru ; Takata, Norio ; Tanaka, Kenji. / Ventrolateral striatal medium spiny neurons positively regulate food-incentive, goal-directed behavior independently of D1 and D2 selectivity. In: Journal of Neuroscience. 2017 ; Vol. 37, No. 10. pp. 2723-2733.
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