Vertically separated anisotropic 3D collagen microchamber array

S. Yoshida, M. Takinoue, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a vertically separated anisotropic collagen hydrogel microchamber array where the boundary could be observed. We dispersed anisotropic collagen/alginate microparticles into microchambers and dissolved only the alginate hydrogel networks. As the result, remaining anisotropic collagen spread at the bottom of each microchamber with keeping the boundary vertical. We successfully demonstrated to observe encapsulated adherent cells in each side and the clear boundary between differently labeled collagen. Our microchamber could be powerful tools for in vitro 3D tissue models, which require to investigate the influence of the boundary of 2 types of ECMs on cell behavior.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages7-8
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16/10/916/10/13

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Keywords

  • 3-dimensional cell culture
  • Anisotropic microenvironment
  • Collagen
  • Microchamber

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Yoshida, S., Takinoue, M., & Onoe, H. (2016). Vertically separated anisotropic 3D collagen microchamber array. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 7-8). Chemical and Biological Microsystems Society.