Abstract
This paper describes a vertically separated anisotropic collagen hydrogel microchamber array where the boundary could be observed. We dispersed anisotropic collagen/alginate microparticles into microchambers and dissolved only the alginate hydrogel networks. As the result, remaining anisotropic collagen spread at the bottom of each microchamber with keeping the boundary vertical. We successfully demonstrated to observe encapsulated adherent cells in each side and the clear boundary between differently labeled collagen. Our microchamber could be powerful tools for in vitro 3D tissue models, which require to investigate the influence of the boundary of 2 types of ECMs on cell behavior.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 7-8 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| Publication status | Published - 2016 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13 |
Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|---|
| Country/Territory | Ireland |
| City | Dublin |
| Period | 16/10/9 → 16/10/13 |
Keywords
- 3-dimensional cell culture
- Anisotropic microenvironment
- Collagen
- Microchamber
ASJC Scopus subject areas
- Control and Systems Engineering