Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates

Toshiyuki Usami, Takeshi Kanto, Patrik Inderbitzin, Mizuho Itoh, Gan Kisaki, Yoshiyuki Ebihara, Wataru Suda, Yoshimiki Amemiya, Krishna V. Subbarao

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In January 2002, Verticillium wilt of lettuce (Lactuca sativa L.) caused by Verticillium tricorpus occurred in upland paddy fields in Hyogo Prefecture for the first time in Japan. This fungal species was first isolated from lettuce in California, USA. In the present study, the genetic relationships between the American and Japanese isolates of V. tricorpus from lettuce were analyzed to determine whether the pathogen could have migrated to Japan from the USA, the major lettuce-seed supplier for Japan. Nucleotide sequences of the rDNA internal transcribed spacer regions, as well as the genes coding for translation elongation factor 1-alpha and RNA polymerase II were compared among American and Japanese V. tricorpus isolates from lettuce. The Japanese isolates of V. tricorpus were distinct from the American. Random amplified polymorphic DNA analyses also supported this conclusion. These results demonstrated that Verticillium wilt on lettuce caused by V. tricorpus in Japan was not related to the isolates causing the disease in California.

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalJournal of General Plant Pathology
Volume77
Issue number1
DOIs
Publication statusPublished - 2011
Externally publishedYes

Fingerprint

Verticillium
lettuce
Japan
Verticillium wilt
random amplified polymorphic DNA technique
Lactuca sativa
DNA-directed RNA polymerase
translation (genetics)
internal transcribed spacers
paddies
genetic relationships
highlands
nucleotide sequences
pathogens
seeds
genes

Keywords

  • Lettuce
  • Phylogenetic analysis
  • Random amplified polymorphic DNA (RAPD)
  • Verticillium tricorpus
  • Verticillium wilt

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Usami, T., Kanto, T., Inderbitzin, P., Itoh, M., Kisaki, G., Ebihara, Y., ... Subbarao, K. V. (2011). Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates. Journal of General Plant Pathology, 77(1), 17-23. https://doi.org/10.1007/s10327-010-0282-x

Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates. / Usami, Toshiyuki; Kanto, Takeshi; Inderbitzin, Patrik; Itoh, Mizuho; Kisaki, Gan; Ebihara, Yoshiyuki; Suda, Wataru; Amemiya, Yoshimiki; Subbarao, Krishna V.

In: Journal of General Plant Pathology, Vol. 77, No. 1, 2011, p. 17-23.

Research output: Contribution to journalArticle

Usami, T, Kanto, T, Inderbitzin, P, Itoh, M, Kisaki, G, Ebihara, Y, Suda, W, Amemiya, Y & Subbarao, KV 2011, 'Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates', Journal of General Plant Pathology, vol. 77, no. 1, pp. 17-23. https://doi.org/10.1007/s10327-010-0282-x
Usami, Toshiyuki ; Kanto, Takeshi ; Inderbitzin, Patrik ; Itoh, Mizuho ; Kisaki, Gan ; Ebihara, Yoshiyuki ; Suda, Wataru ; Amemiya, Yoshimiki ; Subbarao, Krishna V. / Verticillium tricorpus causing lettuce wilt in Japan differs genetically from California lettuce isolates. In: Journal of General Plant Pathology. 2011 ; Vol. 77, No. 1. pp. 17-23.
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