TY - JOUR
T1 - Vicia faba chromosomal aberration assay
AU - Kanaya, N.
AU - Gill, B. S.
AU - Grover, I. S.
AU - Murin, A.
AU - Osiecka, R.
AU - Sandhu, S. S.
AU - Andersson, H. C.
N1 - Funding Information:
The authors express their deepest appreciation to the US EPA, the IPCS, and UNEP for their financial support towards meeting expenses of this collaborative study, and to the US National Institute of Environmental Health Sciences for the supply of chemicals, to Dr. William F. Grant of McGill University for his assistance in preparing the manuscript.
PY - 1994/10/16
Y1 - 1994/10/16
N2 - A collaborative study involving laboratories in six countries was initiated under the sponsorship of the International Programme on Chemical Safety (IPCS) to determine the sensitivity, efficiency and reliability of the Vicia faba root tip meristem chromosomal aberration assay using a standardized protocol. The six Laboratories that participated in this study were located in the Slovak Republic, India, Japan, Poland, Sweden and the USA. All laboratories adhered to a standardized protocol for the Vicia faba chromosomal aberartion assay. Four coded chemicals, azidoglycerol (AG), N-methyl-N-nitrosourea (MNU), sodium azide (NaN3) and maleic hydrazide (MH) were tested with the Vicia faba chromosomal aberration assay. Of the four chemicals, three (MH, AG and MNU) were found to be clastogenic and gave a concentration related response. However, the results of NaN3 were equivocal which might be explained by the stability of NaN3. The conclusions from this study suggest that the Vicia faba chromosomal aberration bioassay is an efficient and reliable short-term bioassay for the rapid screening of chemicals for clastogenicity.
AB - A collaborative study involving laboratories in six countries was initiated under the sponsorship of the International Programme on Chemical Safety (IPCS) to determine the sensitivity, efficiency and reliability of the Vicia faba root tip meristem chromosomal aberration assay using a standardized protocol. The six Laboratories that participated in this study were located in the Slovak Republic, India, Japan, Poland, Sweden and the USA. All laboratories adhered to a standardized protocol for the Vicia faba chromosomal aberartion assay. Four coded chemicals, azidoglycerol (AG), N-methyl-N-nitrosourea (MNU), sodium azide (NaN3) and maleic hydrazide (MH) were tested with the Vicia faba chromosomal aberration assay. Of the four chemicals, three (MH, AG and MNU) were found to be clastogenic and gave a concentration related response. However, the results of NaN3 were equivocal which might be explained by the stability of NaN3. The conclusions from this study suggest that the Vicia faba chromosomal aberration bioassay is an efficient and reliable short-term bioassay for the rapid screening of chemicals for clastogenicity.
KW - 3-Azidoglycerol
KW - Chromosomal aberrations
KW - Clastogens
KW - IPCS collaborative study
KW - Maleic hydrazide
KW - N-Methyl-N-nitrosourea
KW - Sodium azide
KW - Vicia faba
UR - http://www.scopus.com/inward/record.url?scp=0028099357&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028099357&partnerID=8YFLogxK
U2 - 10.1016/0027-5107(94)90116-3
DO - 10.1016/0027-5107(94)90116-3
M3 - Article
C2 - 7523894
AN - SCOPUS:0028099357
SN - 0027-5107
VL - 310
SP - 231
EP - 247
JO - Mutation Research
JF - Mutation Research
IS - 2
ER -