TY - JOUR
T1 - Visualization of migration of human cortical neurons generated from induced pluripotent stem cells
AU - Bamba, Yohei
AU - Kanemura, Yonehiro
AU - Okano, Hideyuki
AU - Yamasaki, Mami
N1 - Funding Information:
This study is supported by the Research on Intractable Diseases and the Research on Applying Health Technology, Health and Labour Sciences Research Grants from the Ministry of Health, Labour and Welfare of Japan to M.Y., and the Program for Intractable Disease Research Utilizing Disease-specific iPS Cells funded by the Japan Science and Technology Agency (JST)/Japan Agency for Medical Research and Development (A-MED) to H.O.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - Background Neuronal migration is considered a key process in human brain development. However, direct observation of migrating human cortical neurons in the fetal brain is accompanied by ethical concerns and is a major obstacle in investigating human cortical neuronal migration. New method We established a novel system that enables direct visualization of migrating cortical neurons generated from human induced pluripotent stem cells (hiPSCs). Results We observed the migration of cortical neurons generated from hiPSCs derived from a control and from a patient with lissencephaly. Methods Our system needs no viable brain tissue, which is usually used in slice culture. Migratory behavior of human cortical neuron can be observed more easily and more vividly by its fluorescence and glial scaffold than that by earlier methods. Conclusions Our in vitro experimental system provides a new platform for investigating development of the human central nervous system and brain malformation.
AB - Background Neuronal migration is considered a key process in human brain development. However, direct observation of migrating human cortical neurons in the fetal brain is accompanied by ethical concerns and is a major obstacle in investigating human cortical neuronal migration. New method We established a novel system that enables direct visualization of migrating cortical neurons generated from human induced pluripotent stem cells (hiPSCs). Results We observed the migration of cortical neurons generated from hiPSCs derived from a control and from a patient with lissencephaly. Methods Our system needs no viable brain tissue, which is usually used in slice culture. Migratory behavior of human cortical neuron can be observed more easily and more vividly by its fluorescence and glial scaffold than that by earlier methods. Conclusions Our in vitro experimental system provides a new platform for investigating development of the human central nervous system and brain malformation.
KW - Cerebral organoid
KW - Cortical neuron
KW - Induced pluripotent stem cells
KW - Radial migration
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U2 - 10.1016/j.jneumeth.2017.07.004
DO - 10.1016/j.jneumeth.2017.07.004
M3 - Article
C2 - 28694214
AN - SCOPUS:85023607014
SN - 0165-0270
VL - 289
SP - 57
EP - 63
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
ER -