Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry

Yuki Sugiura, Yoshiyuki Konishi, Nobuhiro Zaima, Shigeki Kajihara, Hiroki Nakanishi, Ryo Taguchi, Mitsutoshi Setou

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

Previous studies have shown that MALDI-imaging mass spectrometry (IMS) can be used to visualize the distribution of various biomolecules, especially lipids, in the cells and tissues. In this study, we report the cell-selective distribution of PUFA-containing glycerophospholipids (GPLs) in the mouse brain. We established a practical experimental procedure for the IMS of GPLs. We demonstrated that optimization of the composition of the matrix solution and spectrum normalization to the total ion current (TIC) is critical. Using our procedure, we simultaneously differentiated and visualized the localizations of specific molecular species of GPLs in mouse brain sections. The results showed that PUFA-containing phosphatidylcholines (PCs) were distributed in a cell-selective manner: arachidonic acid- and docosahexaenoic acid-containing PCs were seen in the hippocampal neurons and cerebellar Purkinje cells, respectively. Furthermore, these characteristic localizations of PUFA-PCs were formed during neuronal maturation. The phenomenon of brain cell-selective production of specific PUFA-GPLs will help elucidate the potential physiological functions of PUFAs in specific brain regions.

Original languageEnglish
Pages (from-to)1776-1788
Number of pages13
JournalJournal of Lipid Research
Volume50
Issue number9
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Glycerophospholipids
Phosphatidylcholines
Neuroimaging
Mass spectrometry
Brain
Mass Spectrometry
Visualization
Imaging techniques
Docosahexaenoic Acids
Purkinje Cells
Critical currents
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Biomolecules
Arachidonic Acid
Neurons
Ions
Tissue
Lipids
Chemical analysis

Keywords

  • Development
  • Imaging MS
  • LC ESI-MS/MS
  • MALDI
  • Phospholipids

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry. / Sugiura, Yuki; Konishi, Yoshiyuki; Zaima, Nobuhiro; Kajihara, Shigeki; Nakanishi, Hiroki; Taguchi, Ryo; Setou, Mitsutoshi.

In: Journal of Lipid Research, Vol. 50, No. 9, 2009, p. 1776-1788.

Research output: Contribution to journalArticle

Sugiura, Yuki ; Konishi, Yoshiyuki ; Zaima, Nobuhiro ; Kajihara, Shigeki ; Nakanishi, Hiroki ; Taguchi, Ryo ; Setou, Mitsutoshi. / Visualization of the cell-selective distribution of PUFA-containing phosphatidylcholines in mouse brain by imaging mass spectrometry. In: Journal of Lipid Research. 2009 ; Vol. 50, No. 9. pp. 1776-1788.
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