Visualization of the entire differentiation process of murine M cells

Suppression of their maturation in cecal patches

Shunsuke Kimura, M. Yamakami-Kimura, Y. Obata, Kouji Hase, H. Kitamura, H. Ohno, T. Iwanaga

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The microfold (M) cell residing in the follicle-associated epithelium is a specialized epithelial cell that initiates mucosal immune responses by sampling luminal antigens. The differentiation process of M cells remains unclear due to limitations of analytical methods. Here we found that M cells were classified into two functionally different subtypes based on the expression of Glycoprotein 2 (GP2) by newly developed image cytometric analysis. GP2-high M cells actively took up luminal microbeads, whereas GP2-negative or low cells scarcely ingested them, even though both subsets equally expressed the other M-cell signature genes, suggesting that GP2-high M cells represent functionally mature M cells. Further, the GP2-high mature M cells were abundant in Peyer's patch but sparse in the cecal patch: this was most likely due to a decrease in the nuclear translocation of RelB, a downstream transcription factor for the receptor activator of nuclear factor-κB signaling. Given that murine cecum contains a protrusion of beneficial commensals, the restriction of M-cell activity might contribute to preventing the onset of any excessive immune response to the commensals through decelerating the M-cell-dependent uptake of microorganisms.

Original languageEnglish
Pages (from-to)650-660
Number of pages11
JournalMucosal Immunology
Volume8
Issue number3
DOIs
Publication statusPublished - 2015 May 8

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Glycoproteins
Peyer's Patches
Mucosal Immunity
Cecum
Cytoplasmic and Nuclear Receptors
Microspheres
Transcription Factors
Epithelium
Epithelial Cells
Antigens
Genes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Visualization of the entire differentiation process of murine M cells : Suppression of their maturation in cecal patches. / Kimura, Shunsuke; Yamakami-Kimura, M.; Obata, Y.; Hase, Kouji; Kitamura, H.; Ohno, H.; Iwanaga, T.

In: Mucosal Immunology, Vol. 8, No. 3, 08.05.2015, p. 650-660.

Research output: Contribution to journalArticle

Kimura, Shunsuke ; Yamakami-Kimura, M. ; Obata, Y. ; Hase, Kouji ; Kitamura, H. ; Ohno, H. ; Iwanaga, T. / Visualization of the entire differentiation process of murine M cells : Suppression of their maturation in cecal patches. In: Mucosal Immunology. 2015 ; Vol. 8, No. 3. pp. 650-660.
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