Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope

Takahiro Harada, Akiko Kubo, Yuki Sugiura, Nobuhiro Zaima, Takahiro Hayasaka, Naoko Goto-Inoue, Masatoshi Wakui, Makoto Suematsu, Kengo Takeshita, Kiyoshi Ogawa, Yoshikazu Yoshida, Mitsutoshi Setou

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

We have developed a mass microscope (mass spectrometry imager with spatial resolution higher than the naked eye) equipped with an atmospheric pressure ion-source chamber for laser desorption/ionization (AP-LDI) and a quadrupole ion trap time-of-flight (QIT-TOF) analyzer. The optical microscope combined with the mass spectrometer permitted us to precisely determine the relevant tissue region prior to performing imaging mass spectrometry (IMS). An ultraviolet laser tightly focused with a triplet lens was used to achieve high spatial resolution. An atmospheric pressure ion-source chamber enables us to analyze fresh samples with minimal loss of intrinsic water or volatile compounds. Mass-microscopic AP-LDI imaging of freshly cut ginger rhizome sections revealed that 6-gingerol ([M + K]+at m/z 333.15, positive mode; [M - H] - at m/z 293.17, negative mode) and the monoterpene ([M + K] + at m/z 191.09), which are the compounds related to pungency and flavor, respectively, were localized in oil drop-containing organelles. AP-LDI-tandem MS/MS analyses were applied to compare authentic signals from freshly cut ginger directly with the standard reagent. Thus, our atmosphereimaging mass spectrometer enabled us to monitor a quality of plants at the organelle level.

Original languageEnglish
Pages (from-to)9153-9157
Number of pages5
JournalAnalytical Chemistry
Volume81
Issue number21
DOIs
Publication statusPublished - 2009 Nov 1

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Ion sources
Atmospheric pressure
Microscopes
Visualization
Ionization
Desorption
Mass spectrometers
Mass spectrometry
Lasers
Ultraviolet lasers
Imaging techniques
Monoterpenes
Flavors
Image sensors
Lenses
Oils
Ions
Tissue
Water

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope. / Harada, Takahiro; Kubo, Akiko; Sugiura, Yuki; Zaima, Nobuhiro; Hayasaka, Takahiro; Goto-Inoue, Naoko; Wakui, Masatoshi; Suematsu, Makoto; Takeshita, Kengo; Ogawa, Kiyoshi; Yoshida, Yoshikazu; Setou, Mitsutoshi.

In: Analytical Chemistry, Vol. 81, No. 21, 01.11.2009, p. 9153-9157.

Research output: Contribution to journalArticle

Harada, T, Kubo, A, Sugiura, Y, Zaima, N, Hayasaka, T, Goto-Inoue, N, Wakui, M, Suematsu, M, Takeshita, K, Ogawa, K, Yoshida, Y & Setou, M 2009, 'Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope', Analytical Chemistry, vol. 81, no. 21, pp. 9153-9157. https://doi.org/10.1021/ac901872n
Harada, Takahiro ; Kubo, Akiko ; Sugiura, Yuki ; Zaima, Nobuhiro ; Hayasaka, Takahiro ; Goto-Inoue, Naoko ; Wakui, Masatoshi ; Suematsu, Makoto ; Takeshita, Kengo ; Ogawa, Kiyoshi ; Yoshida, Yoshikazu ; Setou, Mitsutoshi. / Visualization of volatile substances in different organelles with an atmospheric-pressure mass microscope. In: Analytical Chemistry. 2009 ; Vol. 81, No. 21. pp. 9153-9157.
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