Visualizing ATP dynamics in live mice

Norimichi Koitabashi, Riki Ogasawara, Ryuto Yasui, Yuki Sugiura, Hinako Matsuda, Shigenori Nonaka, Takashi Izumi, Masahiko Kurabayashi, Makoto Suematsu, Motoko Yanagita, Hiromi Imamura, Masamichi Yamamoto

Research output: Contribution to journalArticlepeer-review


Analysis of the dynamics of adenosine triphosphate (ATP) is vital to quantitatively define the actual roles of ATP in biological activities. Here, we applied a genetically encoded Förster resonance energy transfer biosensor “GO-ATeam” and created a transgenic mouse model that allows systemic ATP levels to be quantitatively, sensitively, noninvasively, and spatiotemporally measured under physiological and pathological conditions. We used this model to readily conduct intravital imaging of ATP dynamics under three different conditions: during exercise, in all organs and cells; during myocardial infarction progression; and in response to the application of cardiotoxic drugs. These findings provide compelling evidence that the GO-ATeam mouse model is a powerful tool to investigate the multifarious functions of cellular ATP in vivo with unprecedented spatiotemporal resolution in real-time. This will inform predictions of molecular and morphological responses to perturbations of ATP levels, as well as the elucidation of physiological mechanisms that control ATP homeostasis.

Original languageEnglish
JournalUnknown Journal
Publication statusPublished - 2020 Jun 10


  • ATP
  • Cardiotoxicity
  • Dynamics
  • Energy metabolism
  • FRET
  • Imaging
  • Inter-organ network
  • Intravital
  • Mice
  • Muscle Contraction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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