Visualizing Trimming Dependence of Biodistribution and Kinetics with Homo-and Heterogeneous N-Glycoclusters on Fluorescent Albumin

Akihiro Ogura, Tsuyoshi Tahara, Satoshi Nozaki, Koji Morimoto, Yasuhiko Kizuka, Shinobu Kitazume, Mitsuko Hara, Soichi Kojima, Hirotaka Onoe, Almira Kurbangalieva, Naoyuki Taniguchi, Yasuyoshi Watanabe, Katsunori Tanaka

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26 Citations (Scopus)

Abstract

A series of N-glycans, each sequentially trimmed from biantennary sialoglycans, were homo-or heterogeneously clustered efficiently on fluorescent albumin using a method that combined strain-promoted alkyne-azide cyclization and 6Ï -azaelectrocyclization. Noninvasive in vivo kinetics and dissection analysis revealed, for the first time, a glycan-dependent shift from urinary to gall bladder excretion mediated by sequential trimming of non-reducing end sialic acids. N-glycoalbumins that were trimmed further, in particular, GlcNAc-and hybrid biantennary-terminated congeners, were selectively taken up by sinusoidal endothelial and stellate cells in the liver, which are critical for diagnosis and treatment of liver fibrillation. Our glycocluster strategy can not only reveal the previously unexplored extracellular functions of N-glycan trimming, but will be classified as the newly emerging glycoprobes for diagnostic and therapeutic applications.

Original languageEnglish
Article number21797
JournalScientific reports
Volume6
DOIs
Publication statusPublished - 2016 Feb 23
Externally publishedYes

ASJC Scopus subject areas

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    Ogura, A., Tahara, T., Nozaki, S., Morimoto, K., Kizuka, Y., Kitazume, S., Hara, M., Kojima, S., Onoe, H., Kurbangalieva, A., Taniguchi, N., Watanabe, Y., & Tanaka, K. (2016). Visualizing Trimming Dependence of Biodistribution and Kinetics with Homo-and Heterogeneous N-Glycoclusters on Fluorescent Albumin. Scientific reports, 6, [21797]. https://doi.org/10.1038/srep21797