Vitamin D3 enhances ATRA-mediated neurosteroid biosynthesis in human glioma GI-1 cells

Toshiaki Yagishita, Akira Kushida, Hiroomi Tamura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Emerging evidence indicates that vitamin D (VD) is an important modulator of brain development and function. To investigate whether VD modulates neurosteroid biosynthesis in neural cells, we investigated the effect of VD 3 on steroidogenic gene expression in human glioma GI-1 cells. We found that VD3 enhanced CYP11A1 and 3β-hydroxysteroid dehydrogenase gene expression. The induction of CYP11A1 gene expression by VD3 was dose-and incubation time-dependent. Calcipotriol, a VD 3 receptor (VDR) agonist, also induced CYP11A1 gene expression in GI-1 cells, indicating that VDR is involved in this induction. The induction of progesterone (PROG) de novo synthesis was observed along with the induction of steroidogenic genes by VD3. Furthermore, VD3 enhanced all-trans retinoic acid (ATRA)-induced CYP11A1 gene expression and PROG production. This suggests cooperative regulation of steroidogenic gene expression by the two fat-soluble vitamins, A and D. In addition, a mixed culture of neuronal IMR-32 cells and GI-1 cells treated with ATRA and VD 3 resulted in the induction of PROG-responsive gene expression in the IMR-32 cells. This result shows a paracrine action of PROG that is induced in and released by the GI-1 cells. The relationship between neurological dysfunction associated with VD deficiency and neurosteroid induction by VD is discussed.

Original languageEnglish
Pages (from-to)285-292
Number of pages8
JournalJournal of Biochemistry
Volume152
Issue number3
DOIs
Publication statusPublished - 2012 Sep

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Cholecalciferol
Biosynthesis
Tretinoin
Gene expression
Glioma
Neurotransmitter Agents
Cholesterol Side-Chain Cleavage Enzyme
Vitamin D
Gene Expression
Progesterone
3-Hydroxysteroid Dehydrogenases
Calcitriol Receptors
Vitamin D Deficiency
Gene Expression Regulation
Vitamin A
Modulators
Brain
Genes
Fats

Keywords

  • ATRA
  • CYP11A1
  • glia
  • neurosteroid
  • vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Vitamin D3 enhances ATRA-mediated neurosteroid biosynthesis in human glioma GI-1 cells. / Yagishita, Toshiaki; Kushida, Akira; Tamura, Hiroomi.

In: Journal of Biochemistry, Vol. 152, No. 3, 09.2012, p. 285-292.

Research output: Contribution to journalArticle

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