WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis

Tetsuro Morisaki, Toru Hirota, Shin ichi Iida, Tomotoshi Marumoto, Toshihiro Hara, Yasuyuki Nishiyama, Michio Kawasuzi, Takehisa Hiraoka, Tatsuyuki Mimori, Norie Araki, Ichiro Izawa, Masaki Inagaki, Hideyuki Saya

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613-phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.

Original languageEnglish
Pages (from-to)319-324
Number of pages6
JournalFEBS Letters
Volume529
Issue number2-3
DOIs
Publication statusPublished - 2002 Oct 9
Externally publishedYes

Keywords

  • Cdc2
  • Centrosome
  • Cyclin B
  • LATS1
  • Mitotic kinase
  • WARTS

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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  • Cite this

    Morisaki, T., Hirota, T., Iida, S. I., Marumoto, T., Hara, T., Nishiyama, Y., Kawasuzi, M., Hiraoka, T., Mimori, T., Araki, N., Izawa, I., Inagaki, M., & Saya, H. (2002). WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis. FEBS Letters, 529(2-3), 319-324. https://doi.org/10.1016/S0014-5793(02)03360-4