Water-protein interactions from high-resolution protein crystallography

Masayoshi Nakasako, J. L. Finney, P. Rand, J. B F N Engberts, R. McKendry, G. Zaccai

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

To understand the role of water in life at molecular and atomic levels, structures and interactions at the protein-water interface have been investigated by cryogenic X-ray crystallography. The method enabled a much clearer visualization of definite hydration sites on the protein surface than at ambient temperature. Using the structural models of proteins, including several hydration water molecules, the characteristics in hydration structures were systematically analysed for the amount, the interaction geometries between water molecules and proteins, and the local and global distribution of water molecules on the surface of proteins. The tetrahedral hydrogen-bond geometry of water molecules in bulk solvent was retained at the interface and enabled the extension of a three-dimensional chain connection of a hydrogen-bond network among hydration water molecules and polar protein atoms over the entire surface of proteins. Networks of hydrogen bonds were quite flexible to accommodate and/or to regulate the conformational changes of proteins such as domain motions. The present experimental results may have profound implications in the understanding of the physico-chemical principles governing the dynamics of proteins in an aqueous environment and a discussion of why water is essential to life at a molecular level.

Original languageEnglish
Pages (from-to)1191-1206
Number of pages16
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume359
Issue number1448
DOIs
Publication statusPublished - 2004 Aug 29

Fingerprint

crystallography
Crystallography
protein
Water
Hydration
hydration
Proteins
proteins
water
Molecules
hydrogen
Hydrogen
Hydrogen bonds
Membrane Proteins
surface proteins
Geometry
geometry
X ray crystallography
Structural Models
X Ray Crystallography

Keywords

  • Cryogenic protein crystallography
  • Hydration structures of proteins
  • Hydrogen bond
  • Water

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Water-protein interactions from high-resolution protein crystallography. / Nakasako, Masayoshi; Finney, J. L.; Rand, P.; Engberts, J. B F N; McKendry, R.; Zaccai, G.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 359, No. 1448, 29.08.2004, p. 1191-1206.

Research output: Contribution to journalArticle

Nakasako, Masayoshi ; Finney, J. L. ; Rand, P. ; Engberts, J. B F N ; McKendry, R. ; Zaccai, G. / Water-protein interactions from high-resolution protein crystallography. In: Philosophical Transactions of the Royal Society B: Biological Sciences. 2004 ; Vol. 359, No. 1448. pp. 1191-1206.
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