Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell–Cell Interactions

Koki Kamiya, Yuta Abe, Kosuke Inoue, Toshihisa Osaki, Ryuji Kawano, Norihisa Miki, Shoji Takeuchi

Research output: Contribution to journalArticle

Abstract

Microfluidic systems have been developed for patterning single cells to study cell–cell interactions. However, patterning multiple types of cells to understand heterogeneous cell–cell interactions remains difficult. Here, it is aimed to develop a cell-trapping device to assemble multiple types of cells in the well-controlled order and morphology. This device mainly comprises a parylene sheet for assembling cells and a microcomb for controlling the cell-trapping area. The cell-trapping area is controlled by moving the parylene sheet on an SU-8 microcomb using tweezers. Gentle downward flow is used as a driving force for the cell-trapping. The assembly of cells on a parylene sheet with round and line-shaped apertures is demonstrated. The cell–cell contacts of the trapped cells are then investigated by direct cell–cell transfer of calcein via connexin nanopores. Finally, using the device with a system for controlling the cell-trapping area, three different types of cells in the well-controlled order are assembled. The correct cell order rate obtained using the device is 27.9%, which is higher than that obtained without the sliding parylene system (0.74%). Furthermore, the occurrence of cell–cell contact between the three cell types assembled is verified. This cell-patterning device will be a useful tool for investigating heterogeneous cell–cell interactions.

Original languageEnglish
Article number1701208
JournalAdvanced healthcare materials
Volume7
Issue number6
DOIs
Publication statusPublished - 2018 Mar 21

Fingerprint

Nanopores
Microfluidics
Connexins
Equipment and Supplies
parylene

Keywords

  • cell patterning
  • cell–cell interaction
  • microdevice
  • microfluidics

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell–Cell Interactions. / Kamiya, Koki; Abe, Yuta; Inoue, Kosuke; Osaki, Toshihisa; Kawano, Ryuji; Miki, Norihisa; Takeuchi, Shoji.

In: Advanced healthcare materials, Vol. 7, No. 6, 1701208, 21.03.2018.

Research output: Contribution to journalArticle

Kamiya, Koki ; Abe, Yuta ; Inoue, Kosuke ; Osaki, Toshihisa ; Kawano, Ryuji ; Miki, Norihisa ; Takeuchi, Shoji. / Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell–Cell Interactions. In: Advanced healthcare materials. 2018 ; Vol. 7, No. 6.
@article{77bcb50431b74942bd73364ab640d5af,
title = "Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell–Cell Interactions",
abstract = "Microfluidic systems have been developed for patterning single cells to study cell–cell interactions. However, patterning multiple types of cells to understand heterogeneous cell–cell interactions remains difficult. Here, it is aimed to develop a cell-trapping device to assemble multiple types of cells in the well-controlled order and morphology. This device mainly comprises a parylene sheet for assembling cells and a microcomb for controlling the cell-trapping area. The cell-trapping area is controlled by moving the parylene sheet on an SU-8 microcomb using tweezers. Gentle downward flow is used as a driving force for the cell-trapping. The assembly of cells on a parylene sheet with round and line-shaped apertures is demonstrated. The cell–cell contacts of the trapped cells are then investigated by direct cell–cell transfer of calcein via connexin nanopores. Finally, using the device with a system for controlling the cell-trapping area, three different types of cells in the well-controlled order are assembled. The correct cell order rate obtained using the device is 27.9{\%}, which is higher than that obtained without the sliding parylene system (0.74{\%}). Furthermore, the occurrence of cell–cell contact between the three cell types assembled is verified. This cell-patterning device will be a useful tool for investigating heterogeneous cell–cell interactions.",
keywords = "cell patterning, cell–cell interaction, microdevice, microfluidics",
author = "Koki Kamiya and Yuta Abe and Kosuke Inoue and Toshihisa Osaki and Ryuji Kawano and Norihisa Miki and Shoji Takeuchi",
year = "2018",
month = "3",
day = "21",
doi = "10.1002/adhm.201701208",
language = "English",
volume = "7",
journal = "Advanced healthcare materials",
issn = "2192-2640",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - Well-Controlled Cell-Trapping Systems for Investigating Heterogeneous Cell–Cell Interactions

AU - Kamiya, Koki

AU - Abe, Yuta

AU - Inoue, Kosuke

AU - Osaki, Toshihisa

AU - Kawano, Ryuji

AU - Miki, Norihisa

AU - Takeuchi, Shoji

PY - 2018/3/21

Y1 - 2018/3/21

N2 - Microfluidic systems have been developed for patterning single cells to study cell–cell interactions. However, patterning multiple types of cells to understand heterogeneous cell–cell interactions remains difficult. Here, it is aimed to develop a cell-trapping device to assemble multiple types of cells in the well-controlled order and morphology. This device mainly comprises a parylene sheet for assembling cells and a microcomb for controlling the cell-trapping area. The cell-trapping area is controlled by moving the parylene sheet on an SU-8 microcomb using tweezers. Gentle downward flow is used as a driving force for the cell-trapping. The assembly of cells on a parylene sheet with round and line-shaped apertures is demonstrated. The cell–cell contacts of the trapped cells are then investigated by direct cell–cell transfer of calcein via connexin nanopores. Finally, using the device with a system for controlling the cell-trapping area, three different types of cells in the well-controlled order are assembled. The correct cell order rate obtained using the device is 27.9%, which is higher than that obtained without the sliding parylene system (0.74%). Furthermore, the occurrence of cell–cell contact between the three cell types assembled is verified. This cell-patterning device will be a useful tool for investigating heterogeneous cell–cell interactions.

AB - Microfluidic systems have been developed for patterning single cells to study cell–cell interactions. However, patterning multiple types of cells to understand heterogeneous cell–cell interactions remains difficult. Here, it is aimed to develop a cell-trapping device to assemble multiple types of cells in the well-controlled order and morphology. This device mainly comprises a parylene sheet for assembling cells and a microcomb for controlling the cell-trapping area. The cell-trapping area is controlled by moving the parylene sheet on an SU-8 microcomb using tweezers. Gentle downward flow is used as a driving force for the cell-trapping. The assembly of cells on a parylene sheet with round and line-shaped apertures is demonstrated. The cell–cell contacts of the trapped cells are then investigated by direct cell–cell transfer of calcein via connexin nanopores. Finally, using the device with a system for controlling the cell-trapping area, three different types of cells in the well-controlled order are assembled. The correct cell order rate obtained using the device is 27.9%, which is higher than that obtained without the sliding parylene system (0.74%). Furthermore, the occurrence of cell–cell contact between the three cell types assembled is verified. This cell-patterning device will be a useful tool for investigating heterogeneous cell–cell interactions.

KW - cell patterning

KW - cell–cell interaction

KW - microdevice

KW - microfluidics

UR - http://www.scopus.com/inward/record.url?scp=85044410979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044410979&partnerID=8YFLogxK

U2 - 10.1002/adhm.201701208

DO - 10.1002/adhm.201701208

M3 - Article

C2 - 29369539

AN - SCOPUS:85044410979

VL - 7

JO - Advanced healthcare materials

JF - Advanced healthcare materials

SN - 2192-2640

IS - 6

M1 - 1701208

ER -