Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions

Yusuke Hirose, Mamiko Onuki, Yuri Tenjimbayashi, Mayuko Yamaguchi-Naka, Seiichiro Mori, Nobutaka Tasaka, Toyomi Satoh, Toru Morisada, Takashi Iwata, Tohru Kiyono, Takashi Mimura, Akihiko Sekizawa, Koji Matsumoto, Iwao Kukimoto

Research output: Contribution to journalArticle

Abstract

Recent large-scale genomics studies of human papillomaviruses (HPVs) have shown a high level of genomic variability of HPV16, the most prevalent genotype in HPV-associated malignancies, and provided new insights into the biological and clinical relevance of its genetic variations in cervical cancer development. Here, we performed deep sequencing analyses of the viral genome to explore genetic variations of HPV16 that are prevalent in Japan. A total of 100 complete genome sequences of HPV16 were determined from cervical specimens collected from Japanese women with cervical intraepithelial neoplasia and invasive cervical cancer, or without cervical malignancies. Phylogenetic analyses revealed the variant distribution in the Japanese HPV16 isolates; overall, lineage A was the most prevalent (94.0%), in which sublineage A4 was dominant (52.0%), followed by sublineage A1 (21.0%). The relative risk of sublineage A4 for cervical cancer development was significantly higher compared to sublineages A1/A2/A3 (odds ratio = 6.72, 95% confidence interval = 1.78-28.9). Interestingly, a novel cluster of variants that branched from A1/A2/A3 was observed for the Japanese HPV16 isolates, indicating that unique HPV16 variants are prevalent among Japanese women.

Original languageEnglish
JournalViruses
Volume11
Issue number4
DOIs
Publication statusPublished - 2019 Apr 16

Fingerprint

Human papillomavirus 16
Uterine Cervical Neoplasms
Genome
varespladib methyl
High-Throughput Nucleotide Sequencing
Cervical Intraepithelial Neoplasia
Viral Genome
Genomics
Neoplasms
Japan
Odds Ratio
Genotype
Confidence Intervals

Keywords

  • cervical cancer
  • HPV16
  • papillomavirus
  • variant

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Hirose, Y., Onuki, M., Tenjimbayashi, Y., Yamaguchi-Naka, M., Mori, S., Tasaka, N., ... Kukimoto, I. (2019). Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions. Viruses, 11(4). https://doi.org/10.3390/v11040350

Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions. / Hirose, Yusuke; Onuki, Mamiko; Tenjimbayashi, Yuri; Yamaguchi-Naka, Mayuko; Mori, Seiichiro; Tasaka, Nobutaka; Satoh, Toyomi; Morisada, Toru; Iwata, Takashi; Kiyono, Tohru; Mimura, Takashi; Sekizawa, Akihiko; Matsumoto, Koji; Kukimoto, Iwao.

In: Viruses, Vol. 11, No. 4, 16.04.2019.

Research output: Contribution to journalArticle

Hirose, Y, Onuki, M, Tenjimbayashi, Y, Yamaguchi-Naka, M, Mori, S, Tasaka, N, Satoh, T, Morisada, T, Iwata, T, Kiyono, T, Mimura, T, Sekizawa, A, Matsumoto, K & Kukimoto, I 2019, 'Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions', Viruses, vol. 11, no. 4. https://doi.org/10.3390/v11040350
Hirose, Yusuke ; Onuki, Mamiko ; Tenjimbayashi, Yuri ; Yamaguchi-Naka, Mayuko ; Mori, Seiichiro ; Tasaka, Nobutaka ; Satoh, Toyomi ; Morisada, Toru ; Iwata, Takashi ; Kiyono, Tohru ; Mimura, Takashi ; Sekizawa, Akihiko ; Matsumoto, Koji ; Kukimoto, Iwao. / Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions. In: Viruses. 2019 ; Vol. 11, No. 4.
@article{299fc012c24d4e1d8c529196bae1541d,
title = "Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions",
abstract = "Recent large-scale genomics studies of human papillomaviruses (HPVs) have shown a high level of genomic variability of HPV16, the most prevalent genotype in HPV-associated malignancies, and provided new insights into the biological and clinical relevance of its genetic variations in cervical cancer development. Here, we performed deep sequencing analyses of the viral genome to explore genetic variations of HPV16 that are prevalent in Japan. A total of 100 complete genome sequences of HPV16 were determined from cervical specimens collected from Japanese women with cervical intraepithelial neoplasia and invasive cervical cancer, or without cervical malignancies. Phylogenetic analyses revealed the variant distribution in the Japanese HPV16 isolates; overall, lineage A was the most prevalent (94.0{\%}), in which sublineage A4 was dominant (52.0{\%}), followed by sublineage A1 (21.0{\%}). The relative risk of sublineage A4 for cervical cancer development was significantly higher compared to sublineages A1/A2/A3 (odds ratio = 6.72, 95{\%} confidence interval = 1.78-28.9). Interestingly, a novel cluster of variants that branched from A1/A2/A3 was observed for the Japanese HPV16 isolates, indicating that unique HPV16 variants are prevalent among Japanese women.",
keywords = "cervical cancer, HPV16, papillomavirus, variant",
author = "Yusuke Hirose and Mamiko Onuki and Yuri Tenjimbayashi and Mayuko Yamaguchi-Naka and Seiichiro Mori and Nobutaka Tasaka and Toyomi Satoh and Toru Morisada and Takashi Iwata and Tohru Kiyono and Takashi Mimura and Akihiko Sekizawa and Koji Matsumoto and Iwao Kukimoto",
year = "2019",
month = "4",
day = "16",
doi = "10.3390/v11040350",
language = "English",
volume = "11",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "4",

}

TY - JOUR

T1 - Whole-Genome Analysis of Human Papillomavirus Type 16 Prevalent in Japanese Women with or without Cervical Lesions

AU - Hirose, Yusuke

AU - Onuki, Mamiko

AU - Tenjimbayashi, Yuri

AU - Yamaguchi-Naka, Mayuko

AU - Mori, Seiichiro

AU - Tasaka, Nobutaka

AU - Satoh, Toyomi

AU - Morisada, Toru

AU - Iwata, Takashi

AU - Kiyono, Tohru

AU - Mimura, Takashi

AU - Sekizawa, Akihiko

AU - Matsumoto, Koji

AU - Kukimoto, Iwao

PY - 2019/4/16

Y1 - 2019/4/16

N2 - Recent large-scale genomics studies of human papillomaviruses (HPVs) have shown a high level of genomic variability of HPV16, the most prevalent genotype in HPV-associated malignancies, and provided new insights into the biological and clinical relevance of its genetic variations in cervical cancer development. Here, we performed deep sequencing analyses of the viral genome to explore genetic variations of HPV16 that are prevalent in Japan. A total of 100 complete genome sequences of HPV16 were determined from cervical specimens collected from Japanese women with cervical intraepithelial neoplasia and invasive cervical cancer, or without cervical malignancies. Phylogenetic analyses revealed the variant distribution in the Japanese HPV16 isolates; overall, lineage A was the most prevalent (94.0%), in which sublineage A4 was dominant (52.0%), followed by sublineage A1 (21.0%). The relative risk of sublineage A4 for cervical cancer development was significantly higher compared to sublineages A1/A2/A3 (odds ratio = 6.72, 95% confidence interval = 1.78-28.9). Interestingly, a novel cluster of variants that branched from A1/A2/A3 was observed for the Japanese HPV16 isolates, indicating that unique HPV16 variants are prevalent among Japanese women.

AB - Recent large-scale genomics studies of human papillomaviruses (HPVs) have shown a high level of genomic variability of HPV16, the most prevalent genotype in HPV-associated malignancies, and provided new insights into the biological and clinical relevance of its genetic variations in cervical cancer development. Here, we performed deep sequencing analyses of the viral genome to explore genetic variations of HPV16 that are prevalent in Japan. A total of 100 complete genome sequences of HPV16 were determined from cervical specimens collected from Japanese women with cervical intraepithelial neoplasia and invasive cervical cancer, or without cervical malignancies. Phylogenetic analyses revealed the variant distribution in the Japanese HPV16 isolates; overall, lineage A was the most prevalent (94.0%), in which sublineage A4 was dominant (52.0%), followed by sublineage A1 (21.0%). The relative risk of sublineage A4 for cervical cancer development was significantly higher compared to sublineages A1/A2/A3 (odds ratio = 6.72, 95% confidence interval = 1.78-28.9). Interestingly, a novel cluster of variants that branched from A1/A2/A3 was observed for the Japanese HPV16 isolates, indicating that unique HPV16 variants are prevalent among Japanese women.

KW - cervical cancer

KW - HPV16

KW - papillomavirus

KW - variant

UR - http://www.scopus.com/inward/record.url?scp=85065070268&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065070268&partnerID=8YFLogxK

U2 - 10.3390/v11040350

DO - 10.3390/v11040350

M3 - Article

C2 - 30995759

AN - SCOPUS:85065070268

VL - 11

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 4

ER -