Whole genome association study of rheumatoid arthritis using 27 039 microsatellites

Gen Tamiya, Minori Shinya, Tadashi Imanishi, Tomoki Ikuta, Satoshi Makino, Koichi Okamoto, Koh Furugaki, Toshiko Matsumoto, Shuhei Mano, Satoshi Ando, Yásuyuki Nozaki, Wataru Yukawa, Ryo Nakashige, Daisuke Yamaguchi, Hideo Ishibashi, Manabu Yonekura, Yuu Nakami, Seiken Takayama, Takaho Endo, Takuya Saruwatari & 24 others Masaru Yagura, Yoko Yoshikawa, Kei Fujimoto, Akira Oka, Suenori Chiku, Samuel E V Linsen, Marius J. Giphart, Jerzy K. Kulski, Toru Fukazawa, Hiroshi Hashimoto, Minoru Kimura, Yuuichi Hoshina, Yasuo Suzuki, Tomomitsu Hotta, Joji Mochida, Takatoshi Minezaki, Koichiro Komai, Shunichi Shiozawa, Atsuo Taniguchi, Hisashi Yamanaka, Naoyuki Kamatani, Takashi Gojobori, Seiamak Bahram, Hidetoshi Inoko

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

A major goal of current human genome-wide studies is to identify the genetic basis of complex disorders. However, the availability of an unbiased, reliable, cost efficient and comprehensive methodology to analyze the entire genome for complex disease association is still largely lacking or problematic. Therefore, we have developed a practical and efficient strategy for whole genome association studies of complex diseases by charting the human genome at 100 kb intervals using a collection of 27 039 microsatellites and the DNA pooling method in three successive genomic screens of independent case-control populations. The final step in our methodology consists of fine mapping of the candidate susceptible DNA regions by single nucleotide polymorphisms (SNPs) analysis. This approach was validated upon application to rheumatoid arthritis, a destructive joint disease affecting up to 1% of the population. A total of 47 candidate regions were identified. The top seven loci, withstanding the most stringent statistical tests, were dissected down to individual genes and/or SNPs on four chromosomes, including the previously known 6p21.3-encoded Major Histocompatibility Complex gene, HLA-DRB1. Hence, microsatellite-based genome-wide association analysis complemented by end stage SNP typing provides a new tool for genetic dissection of multifactorial pathologies including common diseases.

Original languageEnglish
Pages (from-to)2305-2321
Number of pages17
JournalHuman Molecular Genetics
Volume14
Issue number16
DOIs
Publication statusPublished - 2005 Aug 15
Externally publishedYes

Fingerprint

Genome-Wide Association Study
Microsatellite Repeats
Single Nucleotide Polymorphism
Rheumatoid Arthritis
Human Genome
HLA-DRB1 Chains
Joint Diseases
DNA
Major Histocompatibility Complex
Population
Genes
Dissection
Chromosomes
Genome
Pathology
Costs and Cost Analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Tamiya, G., Shinya, M., Imanishi, T., Ikuta, T., Makino, S., Okamoto, K., ... Inoko, H. (2005). Whole genome association study of rheumatoid arthritis using 27 039 microsatellites. Human Molecular Genetics, 14(16), 2305-2321. https://doi.org/10.1093/hmg/ddi234

Whole genome association study of rheumatoid arthritis using 27 039 microsatellites. / Tamiya, Gen; Shinya, Minori; Imanishi, Tadashi; Ikuta, Tomoki; Makino, Satoshi; Okamoto, Koichi; Furugaki, Koh; Matsumoto, Toshiko; Mano, Shuhei; Ando, Satoshi; Nozaki, Yásuyuki; Yukawa, Wataru; Nakashige, Ryo; Yamaguchi, Daisuke; Ishibashi, Hideo; Yonekura, Manabu; Nakami, Yuu; Takayama, Seiken; Endo, Takaho; Saruwatari, Takuya; Yagura, Masaru; Yoshikawa, Yoko; Fujimoto, Kei; Oka, Akira; Chiku, Suenori; Linsen, Samuel E V; Giphart, Marius J.; Kulski, Jerzy K.; Fukazawa, Toru; Hashimoto, Hiroshi; Kimura, Minoru; Hoshina, Yuuichi; Suzuki, Yasuo; Hotta, Tomomitsu; Mochida, Joji; Minezaki, Takatoshi; Komai, Koichiro; Shiozawa, Shunichi; Taniguchi, Atsuo; Yamanaka, Hisashi; Kamatani, Naoyuki; Gojobori, Takashi; Bahram, Seiamak; Inoko, Hidetoshi.

In: Human Molecular Genetics, Vol. 14, No. 16, 15.08.2005, p. 2305-2321.

Research output: Contribution to journalArticle

Tamiya, G, Shinya, M, Imanishi, T, Ikuta, T, Makino, S, Okamoto, K, Furugaki, K, Matsumoto, T, Mano, S, Ando, S, Nozaki, Y, Yukawa, W, Nakashige, R, Yamaguchi, D, Ishibashi, H, Yonekura, M, Nakami, Y, Takayama, S, Endo, T, Saruwatari, T, Yagura, M, Yoshikawa, Y, Fujimoto, K, Oka, A, Chiku, S, Linsen, SEV, Giphart, MJ, Kulski, JK, Fukazawa, T, Hashimoto, H, Kimura, M, Hoshina, Y, Suzuki, Y, Hotta, T, Mochida, J, Minezaki, T, Komai, K, Shiozawa, S, Taniguchi, A, Yamanaka, H, Kamatani, N, Gojobori, T, Bahram, S & Inoko, H 2005, 'Whole genome association study of rheumatoid arthritis using 27 039 microsatellites', Human Molecular Genetics, vol. 14, no. 16, pp. 2305-2321. https://doi.org/10.1093/hmg/ddi234
Tamiya, Gen ; Shinya, Minori ; Imanishi, Tadashi ; Ikuta, Tomoki ; Makino, Satoshi ; Okamoto, Koichi ; Furugaki, Koh ; Matsumoto, Toshiko ; Mano, Shuhei ; Ando, Satoshi ; Nozaki, Yásuyuki ; Yukawa, Wataru ; Nakashige, Ryo ; Yamaguchi, Daisuke ; Ishibashi, Hideo ; Yonekura, Manabu ; Nakami, Yuu ; Takayama, Seiken ; Endo, Takaho ; Saruwatari, Takuya ; Yagura, Masaru ; Yoshikawa, Yoko ; Fujimoto, Kei ; Oka, Akira ; Chiku, Suenori ; Linsen, Samuel E V ; Giphart, Marius J. ; Kulski, Jerzy K. ; Fukazawa, Toru ; Hashimoto, Hiroshi ; Kimura, Minoru ; Hoshina, Yuuichi ; Suzuki, Yasuo ; Hotta, Tomomitsu ; Mochida, Joji ; Minezaki, Takatoshi ; Komai, Koichiro ; Shiozawa, Shunichi ; Taniguchi, Atsuo ; Yamanaka, Hisashi ; Kamatani, Naoyuki ; Gojobori, Takashi ; Bahram, Seiamak ; Inoko, Hidetoshi. / Whole genome association study of rheumatoid arthritis using 27 039 microsatellites. In: Human Molecular Genetics. 2005 ; Vol. 14, No. 16. pp. 2305-2321.
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AU - Yoshikawa, Yoko

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AU - Linsen, Samuel E V

AU - Giphart, Marius J.

AU - Kulski, Jerzy K.

AU - Fukazawa, Toru

AU - Hashimoto, Hiroshi

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AU - Hoshina, Yuuichi

AU - Suzuki, Yasuo

AU - Hotta, Tomomitsu

AU - Mochida, Joji

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