Wisteria floribunda agglutinin-positive mucin 1 is a sensitive biliary marker for human cholangiocarcinoma

Atsushi Matsuda, Atsushi Kuno, Toru Kawamoto, Hideki Matsuzaki, Tatsuro Irimura, Yuzuru Ikehara, Yoh Zen, Yasuni Nakanuma, Masakazu Yamamoto, Nobuhiro Ohkohchi, Junichi Shoda, Jun Hirabayashi, Hisashi Narimatsu

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Abstract

Cholangiocarcinoma (CC) is an aggressive malignant tumor for which useful markers are not presently available for early and precise diagnosis. The aim of this study was therefore to identify a high-performance diagnostic marker with a special focus on glyco-alteration of glycoproteins. In the course of study, we found that Wisteria floribunda agglutinin (WFA) is the best probe to differentiate intrahepatic cholangiocarcinoma (ICC) lesions from normal bile duct epithelia (BDE) (P < 0.0001). The subsequent histochemical study confirmed ICC-specific WFA staining on 165 tissue specimens. On the other hand, the WFA staining was shown to be closely associated with that of MY.1E12 established previously against sialylated mucin 1 (MUC1) by double-staining experiments. Moreover, glyco-alteration of MUC1 could be verified by western blotting of WFA-captured bile samples from patients with CC patients. Thus, we attempted to construct an enzyme-linked immunosorbent assay system for more convenient CC diagnosis, where WFA-coated plates, the specific monoclonal antibody MY.1E12, and the bile specimens from CC including ICC (n = 30) and benign diseases (n = 38) were combined. As a result, CC was clearly distinguished from benign diseases with statistical scores (sensitivity = 90.0%, specificity = 76.3%, and area under the curve = 0.85). As a particular note, the obtained sensitivity is the highest score among those having been so far reported. Conclusion: Our approach focusing significant glyco-alteration of a particular glycoprotein yielded a novel diagnostic system for CC with satisfactory clinical scores.

Original languageEnglish
Pages (from-to)174-182
Number of pages9
JournalHepatology
Volume52
Issue number1
DOIs
Publication statusPublished - 2010 Jul
Externally publishedYes

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Mucin-1
Cholangiocarcinoma
Staining and Labeling
Bile
Glycoproteins
wisteria lectin
Bile Ducts
Area Under Curve
Early Diagnosis
Epithelium
Western Blotting
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Sensitivity and Specificity

ASJC Scopus subject areas

  • Hepatology

Cite this

Matsuda, A., Kuno, A., Kawamoto, T., Matsuzaki, H., Irimura, T., Ikehara, Y., ... Narimatsu, H. (2010). Wisteria floribunda agglutinin-positive mucin 1 is a sensitive biliary marker for human cholangiocarcinoma. Hepatology, 52(1), 174-182. https://doi.org/10.1002/hep.23654

Wisteria floribunda agglutinin-positive mucin 1 is a sensitive biliary marker for human cholangiocarcinoma. / Matsuda, Atsushi; Kuno, Atsushi; Kawamoto, Toru; Matsuzaki, Hideki; Irimura, Tatsuro; Ikehara, Yuzuru; Zen, Yoh; Nakanuma, Yasuni; Yamamoto, Masakazu; Ohkohchi, Nobuhiro; Shoda, Junichi; Hirabayashi, Jun; Narimatsu, Hisashi.

In: Hepatology, Vol. 52, No. 1, 07.2010, p. 174-182.

Research output: Contribution to journalArticle

Matsuda, A, Kuno, A, Kawamoto, T, Matsuzaki, H, Irimura, T, Ikehara, Y, Zen, Y, Nakanuma, Y, Yamamoto, M, Ohkohchi, N, Shoda, J, Hirabayashi, J & Narimatsu, H 2010, 'Wisteria floribunda agglutinin-positive mucin 1 is a sensitive biliary marker for human cholangiocarcinoma', Hepatology, vol. 52, no. 1, pp. 174-182. https://doi.org/10.1002/hep.23654
Matsuda, Atsushi ; Kuno, Atsushi ; Kawamoto, Toru ; Matsuzaki, Hideki ; Irimura, Tatsuro ; Ikehara, Yuzuru ; Zen, Yoh ; Nakanuma, Yasuni ; Yamamoto, Masakazu ; Ohkohchi, Nobuhiro ; Shoda, Junichi ; Hirabayashi, Jun ; Narimatsu, Hisashi. / Wisteria floribunda agglutinin-positive mucin 1 is a sensitive biliary marker for human cholangiocarcinoma. In: Hepatology. 2010 ; Vol. 52, No. 1. pp. 174-182.
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AU - Narimatsu, Hisashi

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