Wisteria floribunda agglutinin-sialylated mucin core polypeptide 1 is a sensitive biomarker for biliary tract carcinoma and intrahepatic cholangiocarcinoma: a multicenter study

Junichi Shoda, Atsushi Matsuda, Takashi Shida, Masakazu Yamamoto, Masato Nagino, Toshio Tsuyuguchi, Takahiro Yasaka, Susumu Tazuma, Kazuhisa Uchiyama, Michiaki Unno, Nobuaki Ohkohchi, Yasuni Nakanuma, Atsushi Kuno, Hisashi Narimatsu

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Abstract

Background: Wisteria floribunda agglutinin (WFA)-sialylated mucin core polypeptide 1 (MUC1) was investigated as a new glycoprotein marker for cholangiocarcinoma (CC) using glycoproteomics technologies. In this multicenter study, WFA-sialylated MUC1 levels in serum and bile samples were measured to determine their diagnostic capability in biliary tract carcinoma (BTC) and intrahepatic (Ih) CC. Methods: The study included 244 patients with BTC, 59 patients with IhCC, 287 patients with benign biliary tract diseases, and 44 control subjects. Results: Serum WFA-sialylated MUC1 levels were significantly higher in patients with either BTC or IhCC than in control subjects and those with benign biliary tract diseases. Patients with IhCC showed higher WFA-sialylated MUC1 levels than patients with tumors at other sites. No significant differences in WFA-sialylated MUC1 levels were found with regard to cancer stage or tissue type. Receiver operating characteristic curve analysis showed that WFA-sialylated MUC1 was superior to carbohydrate antigen 19-9 (CA19-9) and carcinoembryonic antigen (CEA) for the diagnosis of benign biliary tract diseases, BTC, and IhCC, as well as for stage I and II carcinomas. Significantly higher levels of biliary WFA-sialylated MUC1 were observed in BTC/IhCC than in benign biliary tract diseases. The diagnostic capability of biliary WFA-sialylated MUC1 was also superior to that of CA19-9, and diagnostic sensitivity was higher than that of biliary cytology for BTC/IhCC. Conclusions: WFA-sialylated MUC1 is a useful novel biomarker for BTC/IhCC. In the future, this measurement should be applied in the clinical setting.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalJournal of Gastroenterology
DOIs
Publication statusAccepted/In press - 2016 Jun 29
Externally publishedYes

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Keywords

  • Biliary tract carcinoma
  • Biomarker
  • Glycoproteomics
  • Intrahepatic cholangiocarcinoma
  • Multicenter study

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Shoda, J., Matsuda, A., Shida, T., Yamamoto, M., Nagino, M., Tsuyuguchi, T., Yasaka, T., Tazuma, S., Uchiyama, K., Unno, M., Ohkohchi, N., Nakanuma, Y., Kuno, A., & Narimatsu, H. (Accepted/In press). Wisteria floribunda agglutinin-sialylated mucin core polypeptide 1 is a sensitive biomarker for biliary tract carcinoma and intrahepatic cholangiocarcinoma: a multicenter study. Journal of Gastroenterology, 1-11. https://doi.org/10.1007/s00535-016-1230-0