'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells

Kazuma Okamoto, Shunichiro Miyoshi, Masashi Toyoda, Naoko Hida, Yukinori Ikegami, Hatsune Makino, Nobuhiro Nishiyama, Hiroko Tsuji, Chang Hao Cui, Kaoru Segawa, Taro Uyama, Daisuke Kami, Kenji Miyado, Hironori Asada, Kenji Matsumoto, Hirohisa Saito, Yasunori Yoshimura, Satoshi Ogawa, Ryo Aeba, Ryohei YozuAkihiro Umezawa

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The clinical application of cell transplantation for severe heart failure is a promising strategy to improve impaired cardiac function. Recently, an array of cell types, including bone marrow cells, endothelial progenitors, mesenchymal stem cells, resident cardiac stem cells, and embryonic stem cells, have become important candidates for cell sources for cardiac repair. In the present study, we focused on the placenta as a cell source. Cells from the chorionic plate in the fetal portion of the human placenta were obtained after delivery by the primary culture method, and the cells generated in this study had the Y sex chromosome, indicating that the cells were derived from the fetus. The cells potentially expressed 'working' cardiomyocyte-specific genes such as cardiac myosin heavy chain 7β, atrial myosin light chain, cardiac α-actin by gene chip analysis, and Csx/Nkx2.5, GATA4 by RT-PCR, cardiac troponin-I and connexin 43 by immunohistochemistry. These cells were able to differentiate into cardiomyocytes. Cardiac troponin-I and connexin 43 displayed a discontinuous pattern of localization at intercellular contact sites after cardiomyogenic differentiation, suggesting that the chorionic mesoderm contained a large number of cells with cardiomyogenic potential. The cells began spontaneously beating 3 days after co-cultivation with murine fetal cardiomyocytes and the frequency of beating cells reached a maximum on day 10. The contraction of the cardiomyocytes was rhythmical and synchronous, suggesting the presence of electrical communication between the cells. Placenta-derived human fetal cells may be useful for patients who cannot supply bone marrow cells but want to receive stem cell-based cardiac therapy.

Original languageEnglish
Pages (from-to)2550-2562
Number of pages13
JournalExperimental Cell Research
Volume313
Issue number12
DOIs
Publication statusPublished - 2007 Jul 15

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Cardiac Myocytes
Placenta
Action Potentials
Connexin 43
Troponin I
Bone Marrow Cells
Atrial Myosins
Stem Cells
Cardiac Myosins
Myosin Light Chains
Sex Chromosomes
Primary Cell Culture
Myosin Heavy Chains
Y Chromosome
Cell Transplantation
Mesoderm
Embryonic Stem Cells
Oligonucleotide Array Sequence Analysis
Mesenchymal Stromal Cells
Cell Communication

Keywords

  • Cardiac differentiation
  • Co-culture
  • Placenta

ASJC Scopus subject areas

  • Cell Biology

Cite this

'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells. / Okamoto, Kazuma; Miyoshi, Shunichiro; Toyoda, Masashi; Hida, Naoko; Ikegami, Yukinori; Makino, Hatsune; Nishiyama, Nobuhiro; Tsuji, Hiroko; Cui, Chang Hao; Segawa, Kaoru; Uyama, Taro; Kami, Daisuke; Miyado, Kenji; Asada, Hironori; Matsumoto, Kenji; Saito, Hirohisa; Yoshimura, Yasunori; Ogawa, Satoshi; Aeba, Ryo; Yozu, Ryohei; Umezawa, Akihiro.

In: Experimental Cell Research, Vol. 313, No. 12, 15.07.2007, p. 2550-2562.

Research output: Contribution to journalArticle

Okamoto, K, Miyoshi, S, Toyoda, M, Hida, N, Ikegami, Y, Makino, H, Nishiyama, N, Tsuji, H, Cui, CH, Segawa, K, Uyama, T, Kami, D, Miyado, K, Asada, H, Matsumoto, K, Saito, H, Yoshimura, Y, Ogawa, S, Aeba, R, Yozu, R & Umezawa, A 2007, ''Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells', Experimental Cell Research, vol. 313, no. 12, pp. 2550-2562. https://doi.org/10.1016/j.yexcr.2007.04.028
Okamoto, Kazuma ; Miyoshi, Shunichiro ; Toyoda, Masashi ; Hida, Naoko ; Ikegami, Yukinori ; Makino, Hatsune ; Nishiyama, Nobuhiro ; Tsuji, Hiroko ; Cui, Chang Hao ; Segawa, Kaoru ; Uyama, Taro ; Kami, Daisuke ; Miyado, Kenji ; Asada, Hironori ; Matsumoto, Kenji ; Saito, Hirohisa ; Yoshimura, Yasunori ; Ogawa, Satoshi ; Aeba, Ryo ; Yozu, Ryohei ; Umezawa, Akihiro. / 'Working' cardiomyocytes exhibiting plateau action potentials from human placenta-derived extraembryonic mesodermal cells. In: Experimental Cell Research. 2007 ; Vol. 313, No. 12. pp. 2550-2562.
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