Writing and reading methodology for biochips with sub-100-nm chemical patterns based on near-field scanning optical microscopy

Yasuhiro Kobayashi, Masaru Sakai, Akio Ueda, Kenichi Maruyama, Toshiharu Saiki, Koji Suzuki

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

This paper demonstrates a writing and reading methodology, which allows both to create and to detect sub-100-nm carboxyl-terminated patterns on light-transmissive quartz substrates by the same instrumental system. Such a technique, capable of creating carboxyl-terminated nanopatterns, offers several benefits for the miniaturization of biochips, since the carboxyl-terminated nanopatterns allow the easy immobilization of biomolecules by amide bond formation. As a consequence, increasingly miniaturized biochips require suitable analytical methods for the detection of nanopatterns. In our approach, carboxyl-terminated nanopatterns of down to 80 nm width were created using a photolabile silane coupling agent and a UV laser coupled to a near-field scanning optical microscope (NSOM). The same NSOM system was then used in a next step to detect the fabricated carboxyl-terminated nanopatterns after modification with a fluorescent label. Furthermore, as a first step towards biochip applications, the successful immobilization of several biomolecules, such as streptavidin, IgG and DNA on carboxyl-terminated nanopatterns was demonstrated. We have shown that our approach has the potential to lead to a new bioanalytical method, which enables one to write and to read biochips on a sub-100-nm scale by the same system. 2008

Original languageEnglish
Pages (from-to)571-576
Number of pages6
JournalAnalytical Sciences
Volume24
Issue number5
DOIs
Publication statusPublished - 2008 May

ASJC Scopus subject areas

  • Analytical Chemistry

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