WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans

C. E. Rocheleau, J. Yasuda, Ho Shin Tae Ho Shin, R. Lin, H. Sawa, Hideyuki Okano, J. R. Priess, R. J. Davis, C. C. Mello

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

During C. elegans development, Wnt/WG signaling is required for differences in cell fate between sister cells born from anterior/posterior divisions. A β-catenin-related gene, wrm-1, and the lit-1 gene are effectors of this signaling pathway and appear to downregulate the activity of POP-1, a TCF/LEF-related protein, in posterior daughter cells. We show here that lit- 1 encodes a serine/threonine protein kinase homolog related to the Drosophila tissue polarity protein Nemo. We demonstrate that the WRM-1 protein binds to LIT-1 in vivo and that WRM-1 can activate the LIT-1 protein kinase when coexpressed in vertebrate tissue culture cells. This activation leads to phosphorylation of POP-1 and to apparent changes in its subcellular localization. Our findings provide evidence for novel regulatory avenues for an evolutionarily conserved Wnt/WG signaling pathway.

Original languageEnglish
Pages (from-to)717-726
Number of pages10
JournalCell
Volume97
Issue number6
DOIs
Publication statusPublished - 1999
Externally publishedYes

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Protein Kinases
Genes
Catenins
Tissue culture
Phosphorylation
Wnt Signaling Pathway
Proteins
Protein-Serine-Threonine Kinases
Drosophila
Vertebrates
Down-Regulation
Cell Culture Techniques
Chemical activation
Tissue

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Rocheleau, C. E., Yasuda, J., Tae Ho Shin, H. S., Lin, R., Sawa, H., Okano, H., ... Mello, C. C. (1999). WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. Cell, 97(6), 717-726. https://doi.org/10.1016/S0092-8674(00)80784-9

WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. / Rocheleau, C. E.; Yasuda, J.; Tae Ho Shin, Ho Shin; Lin, R.; Sawa, H.; Okano, Hideyuki; Priess, J. R.; Davis, R. J.; Mello, C. C.

In: Cell, Vol. 97, No. 6, 1999, p. 717-726.

Research output: Contribution to journalArticle

Rocheleau, CE, Yasuda, J, Tae Ho Shin, HS, Lin, R, Sawa, H, Okano, H, Priess, JR, Davis, RJ & Mello, CC 1999, 'WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans', Cell, vol. 97, no. 6, pp. 717-726. https://doi.org/10.1016/S0092-8674(00)80784-9
Rocheleau, C. E. ; Yasuda, J. ; Tae Ho Shin, Ho Shin ; Lin, R. ; Sawa, H. ; Okano, Hideyuki ; Priess, J. R. ; Davis, R. J. ; Mello, C. C. / WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. In: Cell. 1999 ; Vol. 97, No. 6. pp. 717-726.
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