Abstract
During C. elegans development, Wnt/WG signaling is required for differences in cell fate between sister cells born from anterior/posterior divisions. A β-catenin-related gene, wrm-1, and the lit-1 gene are effectors of this signaling pathway and appear to downregulate the activity of POP-1, a TCF/LEF-related protein, in posterior daughter cells. We show here that lit- 1 encodes a serine/threonine protein kinase homolog related to the Drosophila tissue polarity protein Nemo. We demonstrate that the WRM-1 protein binds to LIT-1 in vivo and that WRM-1 can activate the LIT-1 protein kinase when coexpressed in vertebrate tissue culture cells. This activation leads to phosphorylation of POP-1 and to apparent changes in its subcellular localization. Our findings provide evidence for novel regulatory avenues for an evolutionarily conserved Wnt/WG signaling pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 717-726 |
| Number of pages | 10 |
| Journal | Cell |
| Volume | 97 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans. / Rocheleau, C. E.; Yasuda, J.; Tae Ho Shin, Ho Shin; Lin, R.; Sawa, H.; Okano, Hideyuki; Priess, J. R.; Davis, R. J.; Mello, C. C.
In: Cell, Vol. 97, No. 6, 1999, p. 717-726.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - WRM-1 activates the LIT-1 protein kinase to transduce anterior/posterior polarity signals in C. elegans
AU - Rocheleau, C. E.
AU - Yasuda, J.
AU - Tae Ho Shin, Ho Shin
AU - Lin, R.
AU - Sawa, H.
AU - Okano, Hideyuki
AU - Priess, J. R.
AU - Davis, R. J.
AU - Mello, C. C.
PY - 1999
Y1 - 1999
N2 - During C. elegans development, Wnt/WG signaling is required for differences in cell fate between sister cells born from anterior/posterior divisions. A β-catenin-related gene, wrm-1, and the lit-1 gene are effectors of this signaling pathway and appear to downregulate the activity of POP-1, a TCF/LEF-related protein, in posterior daughter cells. We show here that lit- 1 encodes a serine/threonine protein kinase homolog related to the Drosophila tissue polarity protein Nemo. We demonstrate that the WRM-1 protein binds to LIT-1 in vivo and that WRM-1 can activate the LIT-1 protein kinase when coexpressed in vertebrate tissue culture cells. This activation leads to phosphorylation of POP-1 and to apparent changes in its subcellular localization. Our findings provide evidence for novel regulatory avenues for an evolutionarily conserved Wnt/WG signaling pathway.
AB - During C. elegans development, Wnt/WG signaling is required for differences in cell fate between sister cells born from anterior/posterior divisions. A β-catenin-related gene, wrm-1, and the lit-1 gene are effectors of this signaling pathway and appear to downregulate the activity of POP-1, a TCF/LEF-related protein, in posterior daughter cells. We show here that lit- 1 encodes a serine/threonine protein kinase homolog related to the Drosophila tissue polarity protein Nemo. We demonstrate that the WRM-1 protein binds to LIT-1 in vivo and that WRM-1 can activate the LIT-1 protein kinase when coexpressed in vertebrate tissue culture cells. This activation leads to phosphorylation of POP-1 and to apparent changes in its subcellular localization. Our findings provide evidence for novel regulatory avenues for an evolutionarily conserved Wnt/WG signaling pathway.
UR - http://www.scopus.com/inward/record.url?scp=0033028966&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033028966&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)80784-9
DO - 10.1016/S0092-8674(00)80784-9
M3 - Article
C2 - 10380924
AN - SCOPUS:0033028966
VL - 97
SP - 717
EP - 726
JO - Cell
JF - Cell
SN - 0092-8674
IS - 6
ER -