X-linked immunodeficient mice spontaneously produce lupus-related anti-RNA helicase A autoantibodies, by are resistant to pristane-induced lupus

Minoru Satoh, Akiei Mizutani, Krista M. Behney, Yoshiki Kuroda, Jun Akaogi, Hideo Yoshida, Dina C. Nacionales, Michito Hirakata, Nobutaka Ono, Westley H. Reeves

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Murine lupus can occur spontaneously or be induced by hydrocarbons, such as pristane. Spontaneous disease in MRL and NZB/W F1 mice is suppressed by the xid (X-linked immunodeficiency) mutation, which greatly diminishes T cell-independent type 2 responses as well as the number of peritoneal B1 cells. The present study asked whether lupus induced by i.p. injection of pristane likewise is inhibited by the xid defect. Male CBA/N (xid) mice were refractory to the induction of autoantibodies by pristane, whereas 23% of pristane-treated male CBA/CaJ controls produced anti-nRNP/Sm, -Su and/or -OJ (isoleucyl tRNA synthetase) antibodies. Unexpectedly, 43% (12 of 28) of the xid mice spontaneously produced anti-nuclear antibodies that proved highly specific for the lupus antigen RNA helicase A (RHA). Strikingly, this specificity was absent in CBA/CaJ mice (none of 51). Moreover, pristane treatment suppressed the production of anti-RHA antibodies when administered prior to the onset of autoantibody production, but enhanced anti-RHA levels when given after the onset of autoantibody production, suggesting that pristane interferes with anti-RHA production at an early stage. Large amounts of IgG1 anti-RHA autoantibodies were detected in the sera of xid mice, whereas pristane-induced anti-nRNP/Sm and -Su autoantibodies were almost exclusively IgG2a. Cytokine production within the peritoneal cavity reflected the predominant isotypes: IL-12 and IFN-γ predominated in pristane-treated mice, whereas IL-4 and IL-6 were more predominant in untreated xid mice. The spontaneous production of anti-RHA by xid mice and its suppression by pristane treatment at the level of autoantibody induction supports the idea that lupus autoantibodies may be generated through a variety of mechanisms.

Original languageEnglish
Pages (from-to)1117-1124
Number of pages8
JournalInternational Immunology
Volume15
Issue number9
DOIs
Publication statusPublished - 2003 Sep 1

Fingerprint

RNA Helicases
Autoantibodies
Isoleucine-tRNA Ligase
pristane
Inbred CBA Mouse
Antibodies
Peritoneal Cavity
Interleukin-12
Hydrocarbons
Interleukin-4
Anti-Idiotypic Antibodies
Interleukin-6
Immunoglobulin G
Cytokines
T-Lymphocytes
Antigens

Keywords

  • Autoantibodies
  • Autoimmunity
  • Cytokines
  • Inflammation

ASJC Scopus subject areas

  • Immunology

Cite this

X-linked immunodeficient mice spontaneously produce lupus-related anti-RNA helicase A autoantibodies, by are resistant to pristane-induced lupus. / Satoh, Minoru; Mizutani, Akiei; Behney, Krista M.; Kuroda, Yoshiki; Akaogi, Jun; Yoshida, Hideo; Nacionales, Dina C.; Hirakata, Michito; Ono, Nobutaka; Reeves, Westley H.

In: International Immunology, Vol. 15, No. 9, 01.09.2003, p. 1117-1124.

Research output: Contribution to journalArticle

Satoh, M, Mizutani, A, Behney, KM, Kuroda, Y, Akaogi, J, Yoshida, H, Nacionales, DC, Hirakata, M, Ono, N & Reeves, WH 2003, 'X-linked immunodeficient mice spontaneously produce lupus-related anti-RNA helicase A autoantibodies, by are resistant to pristane-induced lupus', International Immunology, vol. 15, no. 9, pp. 1117-1124. https://doi.org/10.1093/intimm/dxg110
Satoh, Minoru ; Mizutani, Akiei ; Behney, Krista M. ; Kuroda, Yoshiki ; Akaogi, Jun ; Yoshida, Hideo ; Nacionales, Dina C. ; Hirakata, Michito ; Ono, Nobutaka ; Reeves, Westley H. / X-linked immunodeficient mice spontaneously produce lupus-related anti-RNA helicase A autoantibodies, by are resistant to pristane-induced lupus. In: International Immunology. 2003 ; Vol. 15, No. 9. pp. 1117-1124.
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abstract = "Murine lupus can occur spontaneously or be induced by hydrocarbons, such as pristane. Spontaneous disease in MRL and NZB/W F1 mice is suppressed by the xid (X-linked immunodeficiency) mutation, which greatly diminishes T cell-independent type 2 responses as well as the number of peritoneal B1 cells. The present study asked whether lupus induced by i.p. injection of pristane likewise is inhibited by the xid defect. Male CBA/N (xid) mice were refractory to the induction of autoantibodies by pristane, whereas 23{\%} of pristane-treated male CBA/CaJ controls produced anti-nRNP/Sm, -Su and/or -OJ (isoleucyl tRNA synthetase) antibodies. Unexpectedly, 43{\%} (12 of 28) of the xid mice spontaneously produced anti-nuclear antibodies that proved highly specific for the lupus antigen RNA helicase A (RHA). Strikingly, this specificity was absent in CBA/CaJ mice (none of 51). Moreover, pristane treatment suppressed the production of anti-RHA antibodies when administered prior to the onset of autoantibody production, but enhanced anti-RHA levels when given after the onset of autoantibody production, suggesting that pristane interferes with anti-RHA production at an early stage. Large amounts of IgG1 anti-RHA autoantibodies were detected in the sera of xid mice, whereas pristane-induced anti-nRNP/Sm and -Su autoantibodies were almost exclusively IgG2a. Cytokine production within the peritoneal cavity reflected the predominant isotypes: IL-12 and IFN-γ predominated in pristane-treated mice, whereas IL-4 and IL-6 were more predominant in untreated xid mice. The spontaneous production of anti-RHA by xid mice and its suppression by pristane treatment at the level of autoantibody induction supports the idea that lupus autoantibodies may be generated through a variety of mechanisms.",
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AU - Mizutani, Akiei

AU - Behney, Krista M.

AU - Kuroda, Yoshiki

AU - Akaogi, Jun

AU - Yoshida, Hideo

AU - Nacionales, Dina C.

AU - Hirakata, Michito

AU - Ono, Nobutaka

AU - Reeves, Westley H.

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