X-ray structure of the fourth type of archaeal tRNA splicing endonuclease: Insights into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity

Akira Hirata, Kosuke Fujishima, Ryota Yamagami, Takuya Kawamura, Jillian F. Banfield, Akio Kanai, Hiroyuki Hori

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Cleavage of introns from precursor transfer RNAs (tRNAs) by tRNA splicing endonuclease (EndA) is essential for tRNA maturation in Archaea and Eukarya. In the past, archaeal EndAs were classified into three types (α′ 2, α4 and α2β2) according to subunit composition. Recently, we have identified a fourth type of archaeal EndA from an uncultivated archaeon Candidatus Micrarchaeum acidiphilum, referred to as ARMAN-2, which is deeply branched within Euryarchaea. The ARMAN-2 EndA forms an ε2 homodimer and has broad substrate specificity like the α2β2 type EndAs found in Crenarchaea and Nanoarchaea. However, the precise architecture of ARMAN-2 EndA was unknown. Here, we report the crystal structure of the ϵ 2 homodimer of ARMAN-2 EndA. The structure reveals that the ϵ protomer is separated into three novel units (αN, α and βC) fused by two distinct linkers, although the overall structure of ARMAN-2 EndA is similar to those of the other three types of archaeal EndAs. Structural comparison and mutational analyses reveal that an ARMAN-2 type-specific loop (ASL) is involved in the broad substrate specificity and that K161 in the ASL functions as the RNA recognition site. These findings suggest that the broad substrate specificities of ϵ2 and α2β2 EndAs were separately acquired through different evolutionary processes.

Original languageEnglish
Pages (from-to)10554-10566
Number of pages13
JournalNucleic Acids Research
Volume40
Issue number20
DOIs
Publication statusPublished - 2012 Nov

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Archaeal RNA
RNA Splicing
Endonucleases
Substrate Specificity
Transfer RNA
X-Rays
Archaea
Nanoarchaeota
RNA Precursors
Protein Subunits
Eukaryota
Introns
splicing endonuclease
RNA

ASJC Scopus subject areas

  • Genetics

Cite this

X-ray structure of the fourth type of archaeal tRNA splicing endonuclease : Insights into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity. / Hirata, Akira; Fujishima, Kosuke; Yamagami, Ryota; Kawamura, Takuya; Banfield, Jillian F.; Kanai, Akio; Hori, Hiroyuki.

In: Nucleic Acids Research, Vol. 40, No. 20, 11.2012, p. 10554-10566.

Research output: Contribution to journalArticle

Hirata, Akira ; Fujishima, Kosuke ; Yamagami, Ryota ; Kawamura, Takuya ; Banfield, Jillian F. ; Kanai, Akio ; Hori, Hiroyuki. / X-ray structure of the fourth type of archaeal tRNA splicing endonuclease : Insights into the evolution of a novel three-unit composition and a unique loop involved in broad substrate specificity. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 20. pp. 10554-10566.
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