XMtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance

Nathan N. Lee, Venkata R. Chalamcharla, Francisca Reyes-Turcu, Sameet Mehta, Martin Zofall, Vanivilasini Balachandran, Jothy Dhakshnamoorthy, Nitika Taneja, Soichiro Yamanaka, Ming Zhou, Shiv I S Grewal

Research output: Contribution to journalArticle

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Abstract

The regulation of protein-coding and noncoding RNAs is linked to nuclear processes, including chromatin modifications and gene silencing. However, the mechanisms that distinguish RNAs and mediate their functions are poorly understood. We describe a nuclear RNA-processing network in fission yeast with a core module comprising the Mtr4-like protein, Mtl1, and the zinc-finger protein, Red1. The Mtl1-Red1 core promotes degradation of mRNAs and noncoding RNAs and associates with different proteins to assemble heterochromatin via distinct mechanisms. Mtl1 also forms Red1-independent interactions with evolutionarily conserved proteins named Nrl1 and Ctr1, which associate with splicing factors. Whereas Nrl1 targets transcripts with cryptic introns to form heterochromatin at developmental genes and retrotransposons, Ctr1 functions in processing intron-containing telomerase RNA. Together with our discovery of widespread cryptic introns, including in noncoding RNAs, these findings reveal unique cellular strategies for recognizing regulatory RNAs and coordinating their functions in response to developmental and environmental cues.

Original languageEnglish
JournalCell
Volume155
Issue number5
DOIs
Publication statusPublished - 2013 Nov 21

Fingerprint

Nuclear RNA
Heterochromatin
Telomere
Untranslated RNA
Maintenance
Proteins
Introns
Processing
Genes
RNA
Developmental Genes
Retroelements
Schizosaccharomyces
Zinc Fingers
RNA Stability
Gene Silencing
Yeast
Chromatin
Cues
Zinc

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Lee, N. N., Chalamcharla, V. R., Reyes-Turcu, F., Mehta, S., Zofall, M., Balachandran, V., ... Grewal, S. I. S. (2013). XMtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance. Cell, 155(5). https://doi.org/10.1016/j.cell.2013.10.027

XMtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance. / Lee, Nathan N.; Chalamcharla, Venkata R.; Reyes-Turcu, Francisca; Mehta, Sameet; Zofall, Martin; Balachandran, Vanivilasini; Dhakshnamoorthy, Jothy; Taneja, Nitika; Yamanaka, Soichiro; Zhou, Ming; Grewal, Shiv I S.

In: Cell, Vol. 155, No. 5, 21.11.2013.

Research output: Contribution to journalArticle

Lee, NN, Chalamcharla, VR, Reyes-Turcu, F, Mehta, S, Zofall, M, Balachandran, V, Dhakshnamoorthy, J, Taneja, N, Yamanaka, S, Zhou, M & Grewal, SIS 2013, 'XMtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance', Cell, vol. 155, no. 5. https://doi.org/10.1016/j.cell.2013.10.027
Lee NN, Chalamcharla VR, Reyes-Turcu F, Mehta S, Zofall M, Balachandran V et al. XMtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance. Cell. 2013 Nov 21;155(5). https://doi.org/10.1016/j.cell.2013.10.027
Lee, Nathan N. ; Chalamcharla, Venkata R. ; Reyes-Turcu, Francisca ; Mehta, Sameet ; Zofall, Martin ; Balachandran, Vanivilasini ; Dhakshnamoorthy, Jothy ; Taneja, Nitika ; Yamanaka, Soichiro ; Zhou, Ming ; Grewal, Shiv I S. / XMtr4-like protein coordinates nuclear RNA processing for heterochromatin assembly and for telomere maintenance. In: Cell. 2013 ; Vol. 155, No. 5.
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