TY - JOUR
T1 - XSIP1, a member of two-handed zinc finger proteins, induced anterior neural markers in Xenopus laevis animal cap
AU - Eisaki, Akira
AU - Kuroda, Hiroki
AU - Fukui, Akimasa
AU - Asashima, Makoto
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan, and by CREST (Core Research for Evolutional Science and Technology) of the Japan Science and Technology Corporation. We thank Dr. Yuzuru Eto of Central Research Laboratories of Ajinomoto Co. Inc. for providing human recombinant activin A.
PY - 2000/4/29
Y1 - 2000/4/29
N2 - We characterized Xenopus SIP1 (XSIP1), Smad interacting protein, from activin-treated animal caps by differential screening. The XSIP1 is very similar to mouse SIP1 in the protein coding region including the zinc finger domain and homeodomain. The expression pattern was analyzed by RT-PCR and whole mount in situ hybridization. XSIP1 expression was initially restricted to the dorsal marginal zone in the late gastrula and was subsequently expressed at the lateral edge of neural plate and, in the tailbud stage, in the forebrain, neural tube, and eye. Overexpression of XSIP1 at the animal caps resulted in activation of anterior neural markers without mesodermal markers. Ectopic expression of XSIP1 induced enlargement of neural cells and disordered eye formation. In addition to abnormal head phenotypes, many embryos were short-tailed. Our findings suggest that XSIP1 is a transcriptional repressor, which may be involved in the activin-dependent signal pathway. (C) 2000 Academic Press.
AB - We characterized Xenopus SIP1 (XSIP1), Smad interacting protein, from activin-treated animal caps by differential screening. The XSIP1 is very similar to mouse SIP1 in the protein coding region including the zinc finger domain and homeodomain. The expression pattern was analyzed by RT-PCR and whole mount in situ hybridization. XSIP1 expression was initially restricted to the dorsal marginal zone in the late gastrula and was subsequently expressed at the lateral edge of neural plate and, in the tailbud stage, in the forebrain, neural tube, and eye. Overexpression of XSIP1 at the animal caps resulted in activation of anterior neural markers without mesodermal markers. Ectopic expression of XSIP1 induced enlargement of neural cells and disordered eye formation. In addition to abnormal head phenotypes, many embryos were short-tailed. Our findings suggest that XSIP1 is a transcriptional repressor, which may be involved in the activin-dependent signal pathway. (C) 2000 Academic Press.
KW - Neural induction
KW - SIP1
KW - Xenopus laevis
KW - Zinc finger
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U2 - 10.1006/bbrc.2000.2545
DO - 10.1006/bbrc.2000.2545
M3 - Article
C2 - 10777695
AN - SCOPUS:0034728782
SN - 0006-291X
VL - 271
SP - 151
EP - 157
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -