YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation

Takeshi Shimizu, Yasuyuki Osanai, Kenji Tanaka, Manabu Abe, Rie Natsume, Kenji Sakimura, Kazuhiro Ikenaka

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Oligodendrocytes (OLs) are myelinating cells of the central nervous system. Recent studies have shown that mechanical factors influence various cell properties. Mechanical stimuli can be transduced into intracellular biochemical signals through mechanosensors and intracellular mechanotransducers, such as YAP. However, the molecular mechanisms underlying mechanical regulation of OLs by YAP remain unknown. We found that OL morphology and interactions between OLs and neuronal axons were affected by knocking down YAP. Mechanical stretching of OL precursor cells induced nuclear YAP accumulation and assembly of focal adhesion, key platforms for mechanotransduction. Shear stress decreased the number of OL processes, whereas a dominant-negative form of YAP suppressed these effects. To investigate the roles of YAP in postnatal OLs in vivo, we constructed a novel YAP knock-in mouse and found that in vivo overexpression of YAP widely affected OL maturation. These results indicate that YAP regulates OL morphology and maturation in response to mechanical factors. GLIA 2017;65:360–374.

Original languageEnglish
Pages (from-to)360-374
Number of pages15
JournalGLIA
Volume65
Issue number2
DOIs
Publication statusPublished - 2017 Feb 1

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Oligodendroglia
Morphogenesis
Focal Adhesions
Axons
Central Nervous System

Keywords

  • mechanotransduction
  • oligodendrocyte
  • YAP

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience

Cite this

Shimizu, T., Osanai, Y., Tanaka, K., Abe, M., Natsume, R., Sakimura, K., & Ikenaka, K. (2017). YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation. GLIA, 65(2), 360-374. https://doi.org/10.1002/glia.23096

YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation. / Shimizu, Takeshi; Osanai, Yasuyuki; Tanaka, Kenji; Abe, Manabu; Natsume, Rie; Sakimura, Kenji; Ikenaka, Kazuhiro.

In: GLIA, Vol. 65, No. 2, 01.02.2017, p. 360-374.

Research output: Contribution to journalArticle

Shimizu, T, Osanai, Y, Tanaka, K, Abe, M, Natsume, R, Sakimura, K & Ikenaka, K 2017, 'YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation', GLIA, vol. 65, no. 2, pp. 360-374. https://doi.org/10.1002/glia.23096
Shimizu, Takeshi ; Osanai, Yasuyuki ; Tanaka, Kenji ; Abe, Manabu ; Natsume, Rie ; Sakimura, Kenji ; Ikenaka, Kazuhiro. / YAP functions as a mechanotransducer in oligodendrocyte morphogenesis and maturation. In: GLIA. 2017 ; Vol. 65, No. 2. pp. 360-374.
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