Yeast GAL11 protein is a distinctive type transcription factor that enhances basal transcription in vitro

Hiroshi Sakurai, Yoshiki Hiraoka, Toshio Fukasawa

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The yeast auxiliary transcription factor GAL11, a candidate for the coactivator, was partially purified from yeast cells, and its function was characterized in a cell-free transcription system. The partially purified GAL11 protein stimulated basal transcription from the CYC1 core promoter by a factor of 4-5 at the step of preinitiation complex formation. GAL11 protein also enhanced transcription activated by general regulatory factor 1, GAL4-AH, or GAL4-VP16 to the same extent as the basal transcription. Therefore, the apparent potentiation of the activators by GAL11 was attributable to the stimulation of basal transcription. The wild-type GAL11 protein (but not a mutant-type protein) produced in bacteria stimulated transcription as effectively as GAL11 from yeast. These results suggest that GAL11 functions as a positive cofactor of basal and activator-induced transcription in a cell-free transcription system.

Original languageEnglish
Pages (from-to)8382-8386
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number18
Publication statusPublished - 1993 Sep 15

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Fungal Proteins
Transcription Factors
Cell-Free System
Yeasts
Proteins
Mutant Proteins
Bacteria
In Vitro Techniques

Keywords

  • Core promoter
  • GAL4 derivatives
  • General regulatory factor 1
  • Saccharomyces cerevisiae

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Yeast GAL11 protein is a distinctive type transcription factor that enhances basal transcription in vitro. / Sakurai, Hiroshi; Hiraoka, Yoshiki; Fukasawa, Toshio.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 18, 15.09.1993, p. 8382-8386.

Research output: Contribution to journalArticle

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