Yeast Gal11 protein mediates the transcriptional activation signal of two different transacting factors, Gal4 and general regulatory factor I/repressor/activator site binding protein 1/translation upstream factor

M. Nishizawa, Y. Suzuki, Y. Nogi, K. Matsumoto, T. Fukasawa

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)

Abstract

GAL11 was first identified as a gene required for full expression of some of the galactose-inducible genes in the yeast Saccharomyces cerevisiae. A null mutation within the GAL11 locus causes defects in mating, growth on nonfermentable carbon sources, and sporulation of gal11 homozygotes. The mating defect was observed only in MATα gal11 strains. Northern hybridization analysis revealed that a gal11 mutation impaired transcription of α-specific genes (MFα1 and STE3) but not of an a-specific gene (STE2). Furthermore, this mutation reduced expression of the MATα locus, suggesting that a deficiency in MATα1 protein is responsible for the reduced expression of α-specific genes. Since general regulatory factor I (GFRI)/repressor/activator site binding protein 1 (RAP1)/translation upstream factor (TUF) is believed to be an activator of MATα expression, we examined whether PYK1, which is known to be regulated by GRFI/RAP1/TUF, is also affected by the gal11 mutation. It was determined that the level of PYK1 message was significantly lowered by the mutation. The requirement for functional GAL11 in transcriptional activation was bypassed when either the upstream activating sequence of galactose-inducible genes or of PYK1 was placed very close to the TATA box, suggesting that one of the Gal11 protein functions is to mediate the activation signal of Ga14 and GFRI/RAP1/TUF, when the respective binding site is situated at the naturally occurring distance from the TATA box.

Original languageEnglish
Pages (from-to)5373-5377
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number14
DOIs
Publication statusPublished - 1990

Keywords

  • DNA-binding proteins
  • Mating
  • Protein-protein interaction
  • Transcriptional regulation

ASJC Scopus subject areas

  • General

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