Yeast Pho85 kinase is required for proper gene expression during the diauxic shift

Masafumi Nishizawa, Yuki Katou, Katsuhiko Shirahige, Akio Toh-e

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The budding yeast Saccharomyces cerevisiae changes its gene expression profile when environmental nutritional conditions are changed. Protein kinases including cyclic AMP-dependent kinase, Snf1 and Tor kinases play important roles in this process. Pho85 kinase, a member of the yeast cyclin-dependent kinase family, is involved in the regulation of phosphate metabolism and reserve carbohydrates, and thus is implicated to function as a nutrient-sensing kinase. Upon depletion of glucose in the medium, yeast cells undergo a diauxic shift, accompanied by a carbon metabolic pathway shift, stimulation of mitochondrial function and downregulation of ribosome biogenesis and protein synthesis. We analysed the effect of a pho85Δ mutation on the expression profiles of the genes in this process to investigate whether Pho85 kinase participates in the yeast diauxy. We found that, in the absence of PHO85, a majority of mitochondrial genes were not properly induced, that proteasome-related and chaperonin genes were more repressed, and that, when glucose was still present in the medium, a certain class of genes involved in ribosome biogenesis (ribosomal protein and rRNA processing genes) was repressed, whereas those involved in gluconeogenesis and the glyoxylate cycle were induced. We also found that PHO85 is required for proper expression of several metal sensor genes and their regulatory genes. These results suggest that Pho85 is required for proper onset of changes in expression profiles of genes responsible for the diauxic shift.

Original languageEnglish
Pages (from-to)903-918
Number of pages16
JournalYeast
Volume21
Issue number11
DOIs
Publication statusPublished - 2004 Aug

Fingerprint

Transcriptome
Gene expression
Yeast
phosphotransferases (kinases)
Phosphotransferases
Genes
Yeasts
yeasts
Ribosomes
Gene Expression
gene expression
Chaperonins
Glucose
Adenylate Kinase
Saccharomycetales
Mitochondrial Genes
Gluconeogenesis
genes
Cyclin-Dependent Kinases
Ribosomal Proteins

Keywords

  • Cyclin-dependent kinase
  • Diauxic shift
  • Gene expression
  • Metabolism
  • Mitochondrial function

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Nishizawa, M., Katou, Y., Shirahige, K., & Toh-e, A. (2004). Yeast Pho85 kinase is required for proper gene expression during the diauxic shift. Yeast, 21(11), 903-918. https://doi.org/10.1002/yea.1138

Yeast Pho85 kinase is required for proper gene expression during the diauxic shift. / Nishizawa, Masafumi; Katou, Yuki; Shirahige, Katsuhiko; Toh-e, Akio.

In: Yeast, Vol. 21, No. 11, 08.2004, p. 903-918.

Research output: Contribution to journalArticle

Nishizawa, M, Katou, Y, Shirahige, K & Toh-e, A 2004, 'Yeast Pho85 kinase is required for proper gene expression during the diauxic shift', Yeast, vol. 21, no. 11, pp. 903-918. https://doi.org/10.1002/yea.1138
Nishizawa, Masafumi ; Katou, Yuki ; Shirahige, Katsuhiko ; Toh-e, Akio. / Yeast Pho85 kinase is required for proper gene expression during the diauxic shift. In: Yeast. 2004 ; Vol. 21, No. 11. pp. 903-918.
@article{4d948c4b6a474477bdad73445cf88ae6,
title = "Yeast Pho85 kinase is required for proper gene expression during the diauxic shift",
abstract = "The budding yeast Saccharomyces cerevisiae changes its gene expression profile when environmental nutritional conditions are changed. Protein kinases including cyclic AMP-dependent kinase, Snf1 and Tor kinases play important roles in this process. Pho85 kinase, a member of the yeast cyclin-dependent kinase family, is involved in the regulation of phosphate metabolism and reserve carbohydrates, and thus is implicated to function as a nutrient-sensing kinase. Upon depletion of glucose in the medium, yeast cells undergo a diauxic shift, accompanied by a carbon metabolic pathway shift, stimulation of mitochondrial function and downregulation of ribosome biogenesis and protein synthesis. We analysed the effect of a pho85Δ mutation on the expression profiles of the genes in this process to investigate whether Pho85 kinase participates in the yeast diauxy. We found that, in the absence of PHO85, a majority of mitochondrial genes were not properly induced, that proteasome-related and chaperonin genes were more repressed, and that, when glucose was still present in the medium, a certain class of genes involved in ribosome biogenesis (ribosomal protein and rRNA processing genes) was repressed, whereas those involved in gluconeogenesis and the glyoxylate cycle were induced. We also found that PHO85 is required for proper expression of several metal sensor genes and their regulatory genes. These results suggest that Pho85 is required for proper onset of changes in expression profiles of genes responsible for the diauxic shift.",
keywords = "Cyclin-dependent kinase, Diauxic shift, Gene expression, Metabolism, Mitochondrial function",
author = "Masafumi Nishizawa and Yuki Katou and Katsuhiko Shirahige and Akio Toh-e",
year = "2004",
month = "8",
doi = "10.1002/yea.1138",
language = "English",
volume = "21",
pages = "903--918",
journal = "Yeast",
issn = "0749-503X",
publisher = "John Wiley and Sons Ltd",
number = "11",

}

TY - JOUR

T1 - Yeast Pho85 kinase is required for proper gene expression during the diauxic shift

AU - Nishizawa, Masafumi

AU - Katou, Yuki

AU - Shirahige, Katsuhiko

AU - Toh-e, Akio

PY - 2004/8

Y1 - 2004/8

N2 - The budding yeast Saccharomyces cerevisiae changes its gene expression profile when environmental nutritional conditions are changed. Protein kinases including cyclic AMP-dependent kinase, Snf1 and Tor kinases play important roles in this process. Pho85 kinase, a member of the yeast cyclin-dependent kinase family, is involved in the regulation of phosphate metabolism and reserve carbohydrates, and thus is implicated to function as a nutrient-sensing kinase. Upon depletion of glucose in the medium, yeast cells undergo a diauxic shift, accompanied by a carbon metabolic pathway shift, stimulation of mitochondrial function and downregulation of ribosome biogenesis and protein synthesis. We analysed the effect of a pho85Δ mutation on the expression profiles of the genes in this process to investigate whether Pho85 kinase participates in the yeast diauxy. We found that, in the absence of PHO85, a majority of mitochondrial genes were not properly induced, that proteasome-related and chaperonin genes were more repressed, and that, when glucose was still present in the medium, a certain class of genes involved in ribosome biogenesis (ribosomal protein and rRNA processing genes) was repressed, whereas those involved in gluconeogenesis and the glyoxylate cycle were induced. We also found that PHO85 is required for proper expression of several metal sensor genes and their regulatory genes. These results suggest that Pho85 is required for proper onset of changes in expression profiles of genes responsible for the diauxic shift.

AB - The budding yeast Saccharomyces cerevisiae changes its gene expression profile when environmental nutritional conditions are changed. Protein kinases including cyclic AMP-dependent kinase, Snf1 and Tor kinases play important roles in this process. Pho85 kinase, a member of the yeast cyclin-dependent kinase family, is involved in the regulation of phosphate metabolism and reserve carbohydrates, and thus is implicated to function as a nutrient-sensing kinase. Upon depletion of glucose in the medium, yeast cells undergo a diauxic shift, accompanied by a carbon metabolic pathway shift, stimulation of mitochondrial function and downregulation of ribosome biogenesis and protein synthesis. We analysed the effect of a pho85Δ mutation on the expression profiles of the genes in this process to investigate whether Pho85 kinase participates in the yeast diauxy. We found that, in the absence of PHO85, a majority of mitochondrial genes were not properly induced, that proteasome-related and chaperonin genes were more repressed, and that, when glucose was still present in the medium, a certain class of genes involved in ribosome biogenesis (ribosomal protein and rRNA processing genes) was repressed, whereas those involved in gluconeogenesis and the glyoxylate cycle were induced. We also found that PHO85 is required for proper expression of several metal sensor genes and their regulatory genes. These results suggest that Pho85 is required for proper onset of changes in expression profiles of genes responsible for the diauxic shift.

KW - Cyclin-dependent kinase

KW - Diauxic shift

KW - Gene expression

KW - Metabolism

KW - Mitochondrial function

UR - http://www.scopus.com/inward/record.url?scp=4444220038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444220038&partnerID=8YFLogxK

U2 - 10.1002/yea.1138

DO - 10.1002/yea.1138

M3 - Article

VL - 21

SP - 903

EP - 918

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 11

ER -