Zebrafish Dkk1, induced by the pre-MBT Wnt signaling, is secreted from the prechordal plate and patterns the anterior neural plate

Minori Shinya, Cathrin Eschbach, Matthew Clark, Hans Lehrach, Makoto Furutani-Seiki

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

mRNA injection into the ventral blastomeres of Xenopus embryos of mRNA encoding Wnt pathway genes induces a secondary axis with complete head structures. To identify target genes of the pre-MBT dorsalization pathway that might be responsible for head formation in zebrafish, we have cloned zebrafish dickkopf1 (dkk1), which is expressed in tissues implicated in head patterning. We found that dkk1 blocks the post-MBT Wnt signaling and dkk1 is a target of the pre-MBT Wnt signaling. Dkk1 overexpression in the prechordal plate suggests that Dkk1, secreted from the prechordal plate, expands the forebrain at the expense of the midbrain in the anterior neural plate. Furthermore, dkk1 acts in parallel to the homeobox gene bozozok and bozozok is required for the maintenance of dkk1 expression. The nodal gene squint is also required for the maintenance of dkk1 expression. Among the mutually dependent target genes of the pre-MBT Wnt signaling, dkk1 plays an important role in patterning the anterior head of zebrafish. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)3-17
Number of pages15
JournalMechanisms of Development
Volume98
Issue number1-2
DOIs
Publication statusPublished - 2000 Nov 1
Externally publishedYes

Fingerprint

Neural Plate
Zebrafish
Head
Genes
Maintenance
Blastomeres
Messenger RNA
Wnt Signaling Pathway
Homeobox Genes
Strabismus
Prosencephalon
Mesencephalon
Xenopus
Embryonic Structures
Injections

Keywords

  • A-P patterning
  • Anterior neural plate
  • Dkk-1
  • Forebrain
  • Prechordal plate
  • Wnt signaling
  • Zebrafish

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

Cite this

Zebrafish Dkk1, induced by the pre-MBT Wnt signaling, is secreted from the prechordal plate and patterns the anterior neural plate. / Shinya, Minori; Eschbach, Cathrin; Clark, Matthew; Lehrach, Hans; Furutani-Seiki, Makoto.

In: Mechanisms of Development, Vol. 98, No. 1-2, 01.11.2000, p. 3-17.

Research output: Contribution to journalArticle

Shinya, Minori ; Eschbach, Cathrin ; Clark, Matthew ; Lehrach, Hans ; Furutani-Seiki, Makoto. / Zebrafish Dkk1, induced by the pre-MBT Wnt signaling, is secreted from the prechordal plate and patterns the anterior neural plate. In: Mechanisms of Development. 2000 ; Vol. 98, No. 1-2. pp. 3-17.
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