Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress

Wakana Ohashi, Shunsuke Kimura, Toshihiko Iwanaga, Yukihiro Furusawa, Tarou Irié, Hironori Izumi, Takashi Watanabe, Atsushi Hijikata, Takafumi Hara, Osamu Ohara, Haruhiko Koseki, Toshiro Sato, Sylvie Robine, Hisashi Mori, Yuichi Hattori, Hiroshi Watarai, Kenji Mishima, Hiroshi Ohno, Kouji Hase, Toshiyuki Fukada

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Zinc transporters play a critical role in spatiotemporal regulation of zinc homeostasis. Although disruption of zinc homeostasis has been implicated in disorders such as intestinal inflammation and aberrant epithelial morphology, it is largely unknown which zinc transporters are responsible for the intestinal epithelial homeostasis. Here, we show that Zrt-Irt-like protein (ZIP) transporter ZIP7, which is highly expressed in the intestinal crypt, is essential for intestinal epithelial proliferation. Mice lacking Zip7 in intestinal epithelium triggered endoplasmic reticulum (ER) stress in proliferative progenitor cells, leading to significant cell death of progenitor cells. Zip7 deficiency led to the loss of Olfm4+ intestinal stem cells and the degeneration of post-mitotic Paneth cells, indicating a fundamental requirement for Zip7 in homeostatic intestinal regeneration. Taken together, these findings provide evidence for the importance of ZIP7 in maintenance of intestinal epithelial homeostasis through the regulation of ER function in proliferative progenitor cells and maintenance of intestinal stem cells. Therapeutic targeting of ZIP7 could lead to effective treatment of gastrointestinal disorders.

Original languageEnglish
Article numbere1006349
JournalPLoS Genetics
Volume12
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

Fingerprint

Endoplasmic Reticulum Stress
homeostasis
endoplasmic reticulum
transporters
stem cells
Stem Cells
zinc
Homeostasis
stem
Zinc
Maintenance
Paneth Cells
intestinal crypts
targeting
Intestinal Mucosa
intestinal mucosa
digestive system diseases
regeneration
Endoplasmic Reticulum
cell death

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress. / Ohashi, Wakana; Kimura, Shunsuke; Iwanaga, Toshihiko; Furusawa, Yukihiro; Irié, Tarou; Izumi, Hironori; Watanabe, Takashi; Hijikata, Atsushi; Hara, Takafumi; Ohara, Osamu; Koseki, Haruhiko; Sato, Toshiro; Robine, Sylvie; Mori, Hisashi; Hattori, Yuichi; Watarai, Hiroshi; Mishima, Kenji; Ohno, Hiroshi; Hase, Kouji; Fukada, Toshiyuki.

In: PLoS Genetics, Vol. 12, No. 10, e1006349, 01.10.2016.

Research output: Contribution to journalArticle

Ohashi, W, Kimura, S, Iwanaga, T, Furusawa, Y, Irié, T, Izumi, H, Watanabe, T, Hijikata, A, Hara, T, Ohara, O, Koseki, H, Sato, T, Robine, S, Mori, H, Hattori, Y, Watarai, H, Mishima, K, Ohno, H, Hase, K & Fukada, T 2016, 'Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress', PLoS Genetics, vol. 12, no. 10, e1006349. https://doi.org/10.1371/journal.pgen.1006349
Ohashi, Wakana ; Kimura, Shunsuke ; Iwanaga, Toshihiko ; Furusawa, Yukihiro ; Irié, Tarou ; Izumi, Hironori ; Watanabe, Takashi ; Hijikata, Atsushi ; Hara, Takafumi ; Ohara, Osamu ; Koseki, Haruhiko ; Sato, Toshiro ; Robine, Sylvie ; Mori, Hisashi ; Hattori, Yuichi ; Watarai, Hiroshi ; Mishima, Kenji ; Ohno, Hiroshi ; Hase, Kouji ; Fukada, Toshiyuki. / Zinc Transporter SLC39A7/ZIP7 Promotes Intestinal Epithelial Self-Renewal by Resolving ER Stress. In: PLoS Genetics. 2016 ; Vol. 12, No. 10.
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