Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis

Kei ichiro Ishiguro, Manuela Monti, Tomohiko Akiyama, Hiromi Kimura, Nana Chikazawa-Nohtomi, Miki Sakota, Saeko Sato, Carlo Alberto Redi, Shigeru Ko, Minoru Ko

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Mouse zinc finger and SCAN domain containing 4 (Zscan4) proteins, which are encoded by multiple copies of Zscan4 genes, are expressed specifically in preimplantation embryos in vivo and embryonic stem (ES) cells in vitro. However, the expression patterns of mouse Zscan4 in vivo have been largely elusive. Here, we show that Zscan4 proteins are expressed in adult ovaries and testes. In ovaries, Zscan4 proteins were detected in germinal vesicle (GV) stage oocytes in antral follicles, indicating that Zscan4 genes are activated during the diplotene/dictyate stage in meiotic prophase I. Remarkably, Zscan4 showed different spatial localization patterns between two distinct GV oocytes, which can be distinguished by global chromatin organization—surrounded nucleolus (SN) and non-surrounded nucleolus (NSN). These spatiotemporal differences in Zscan4 localizations correlated with the transition of RNA polymerase II-mediated transcriptional status during GV oocyte maturation. In testes, Zscan4 proteins were detected in spermatocytes at late pachytene/diplotene stages and in Sertoli cells. These results suggest that Zscan4 may play critical roles during late meiotic prophase in both males and females.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalIn Vitro Cellular and Developmental Biology - Animal
DOIs
Publication statusAccepted/In press - 2016 Oct 3

Fingerprint

Prophase
Oogenesis
Zinc Fingers
Spermatogenesis
Zinc
Meiotic Prophase I
Oocytes
Testis
Ovary
Proteins
Genes
Pachytene Stage
Spermatocytes
Sertoli Cells
RNA Polymerase II
Blastocyst
Embryonic Stem Cells
Stem cells
Chromatin

Keywords

  • ES cell
  • GV oocyte
  • Ovary
  • Preimplantation embryo
  • SN and NSN oocyte
  • Testis

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis. / Ishiguro, Kei ichiro; Monti, Manuela; Akiyama, Tomohiko; Kimura, Hiromi; Chikazawa-Nohtomi, Nana; Sakota, Miki; Sato, Saeko; Redi, Carlo Alberto; Ko, Shigeru; Ko, Minoru.

In: In Vitro Cellular and Developmental Biology - Animal, 03.10.2016, p. 1-12.

Research output: Contribution to journalArticle

Ishiguro, Kei ichiro ; Monti, Manuela ; Akiyama, Tomohiko ; Kimura, Hiromi ; Chikazawa-Nohtomi, Nana ; Sakota, Miki ; Sato, Saeko ; Redi, Carlo Alberto ; Ko, Shigeru ; Ko, Minoru. / Zscan4 is expressed specifically during late meiotic prophase in both spermatogenesis and oogenesis. In: In Vitro Cellular and Developmental Biology - Animal. 2016 ; pp. 1-12.
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