Zymographic analysis of circulating and tissue forms of colon carcinoma gelatinase A (MMP-2) and B (MMP-9) separated by mono- and two-dimensional electrophoresis

Ida Pucci-Minafra, Salvatore Minafra, Giampiero La Rocca, Marilisa Barranca, Simona Fontana, Giuseppina Alaimo, Yasunori Okada

Research output: Contribution to journalArticle

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Abstract

Gelatinase A (MMP-2) and gelatinase B (MMP-9) play a key role in the proteolytic cascade leading to ECM degradation during invasion and metastasis. The enzyme activity is regulated both at the intra- and extra-cellular level. Extracellular regulation is achieved mainly through the balance between proenzyme activation and inhibition, which appears to be altered in cancer patients. One of the mechanisms of MMP inhibition is the binding of the enzymes to appropriate tissue inhibitors (TIMP). In the recent literature, it has been suggested that MMP-2 and/or MMP-9 are indeed over-produced in many carcinomas, while the identity of the various enzymatic forms (latent, activated and enzyme/inhibitor complexes) remains to be elucidated. In this study we have analyzed the circulating forms of MMP-9 and MMP-2 in serum samples of patients with colon carcinoma, as well as the enzymatic activities present in tissue extracts from surgical fragments (primary tumor and its paired healthy tissue). Proteins were separated by means of mono-dimensional or bidimensional electrophoresis, and the enzymes detected by gelatin zymography and immunological assays. The results of densitometric analyses demonstrate that proMMP-9, but not proMMP-2, is significantly higher in the oncologic sera vs. the normal sera. In addition, several oligomeric circulating and tissue forms of MMP-9 are preferentially found in the oncologic samples, both in mono- and second-dimension zymograms. The activated forms of MMP-2 and MMP-9 are uniquely present in the primary tumor extracts, thus confirming the involvement of the tissue microenvironment in gelatinase activation and function.

Original languageEnglish
Pages (from-to)419-427
Number of pages9
JournalMatrix Biology
Volume20
Issue number7
DOIs
Publication statusPublished - 2001

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Matrix Metalloproteinase 2
Matrix Metalloproteinases
Electrophoresis
Colon
Carcinoma
Enzymes
Serum
Gelatinases
Neoplasms
Enzyme Precursors
Tissue Extracts
Matrix Metalloproteinase 9
Enzyme Inhibitors
Gelatin
Neoplasm Metastasis

Keywords

  • Colon cancer
  • Matrix metalloproteinases
  • Second-dimension zymography

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Zymographic analysis of circulating and tissue forms of colon carcinoma gelatinase A (MMP-2) and B (MMP-9) separated by mono- and two-dimensional electrophoresis. / Pucci-Minafra, Ida; Minafra, Salvatore; La Rocca, Giampiero; Barranca, Marilisa; Fontana, Simona; Alaimo, Giuseppina; Okada, Yasunori.

In: Matrix Biology, Vol. 20, No. 7, 2001, p. 419-427.

Research output: Contribution to journalArticle

Pucci-Minafra, Ida ; Minafra, Salvatore ; La Rocca, Giampiero ; Barranca, Marilisa ; Fontana, Simona ; Alaimo, Giuseppina ; Okada, Yasunori. / Zymographic analysis of circulating and tissue forms of colon carcinoma gelatinase A (MMP-2) and B (MMP-9) separated by mono- and two-dimensional electrophoresis. In: Matrix Biology. 2001 ; Vol. 20, No. 7. pp. 419-427.
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AU - Alaimo, Giuseppina

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