Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor

Toru Hirota, Tetsuro Morisaki, Yasuyuki Nishiyama, Tomotoshi Marumoto, Kenji Tada, Toshihiro Hara, Norio Masuko, Masaki Inagaki, Katsuyoshi Hatakeyama, Hideyuki Saya

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

The mitotic apparatus plays a pivotal role in dividing cells to ensure each daughter cell receives a full set of chromosomes and complement of cytoplasm during mitosis. A human homologue of the Drosophila warts tumor suppressor, h-warts/LATS1, is an evolutionarily conserved serine/threonine kinase and a dynamic component of the mitotic apparatus. We have identified an interaction of h-warts/LATS1 with zyxin, a regulator of actin filament assembly. Zyxin is a component of focal adhesion, however, during mitosis a fraction of cytoplasmic-dispersed zyxin becomes associated with h-warts/LATS1 on the mitotic apparatus. We found that zyxin is phosphorylated specifically during mitosis, most likely by Cdc2 kinase, and that the phosphorylation regulates association with h-warts/LATS1. Furthermore, microinjection of truncated h-warts/LATS1 protein, including the zyxin-binding portion, interfered with localization of zyxin to mitotic apparatus, and the duration of mitosis of these injected cells was significantly longer than that of control cells. These findings suggest that h-warts/LATS1 and zyxin play a crucial role in controlling mitosis progression by forming a regulatory complex on mitotic apparatus.

Original languageEnglish
Pages (from-to)1073-1086
Number of pages14
JournalJournal of Cell Biology
Volume149
Issue number5
DOIs
Publication statusPublished - 2000 May 29
Externally publishedYes

Fingerprint

Zyxin
Spindle Apparatus
Warts
Actin Cytoskeleton
Mitosis
Neoplasms
Focal Adhesions
Protein-Serine-Threonine Kinases
Microinjections
Drosophila
Cytoplasm
Phosphotransferases
Chromosomes
Phosphorylation

Keywords

  • Cdc2
  • Interaction
  • Mitotic spindle
  • Phosphorylation
  • Serine/threonine kinase

ASJC Scopus subject areas

  • Cell Biology

Cite this

Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor. / Hirota, Toru; Morisaki, Tetsuro; Nishiyama, Yasuyuki; Marumoto, Tomotoshi; Tada, Kenji; Hara, Toshihiro; Masuko, Norio; Inagaki, Masaki; Hatakeyama, Katsuyoshi; Saya, Hideyuki.

In: Journal of Cell Biology, Vol. 149, No. 5, 29.05.2000, p. 1073-1086.

Research output: Contribution to journalArticle

Hirota, T, Morisaki, T, Nishiyama, Y, Marumoto, T, Tada, K, Hara, T, Masuko, N, Inagaki, M, Hatakeyama, K & Saya, H 2000, 'Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor', Journal of Cell Biology, vol. 149, no. 5, pp. 1073-1086. https://doi.org/10.1083/jcb.149.5.1073
Hirota, Toru ; Morisaki, Tetsuro ; Nishiyama, Yasuyuki ; Marumoto, Tomotoshi ; Tada, Kenji ; Hara, Toshihiro ; Masuko, Norio ; Inagaki, Masaki ; Hatakeyama, Katsuyoshi ; Saya, Hideyuki. / Zyxin, a regulator of actin filament assembly, targets the mitotic apparatus by interacting with h-warts/LATS1 tumor suppressor. In: Journal of Cell Biology. 2000 ; Vol. 149, No. 5. pp. 1073-1086.
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