Kei Fujiwara

2022

Characterization of the membrane penetration-enhancing peptide S19 derived from human syncytin-1 for the intracellular delivery of TAT-fused proteins

Suzuki, M., Iwaki, K., Kikuchi, M., Fujiwara, K. & Doi, N., 2022 1月 1, In: Biochemical and Biophysical Research Communications. 586, p. 63-67 5 p.

研究成果: Article › 査読

Open Access

2 被引用数 (Scopus)

Controlling the Periodicity of a Reaction-Diffusion Wave in Artificial Cells by a Two-Way Energy Supplier

Takada, S., Yoshinaga, N., Doi, N. & Fujiwara, K., 2022 10月 25, In: ACS Nano. 16, 10, p. 16853-16861 9 p.

研究成果: Article › 査読

Cytoplasmic delivery of siRNA using human-derived membrane penetration-enhancing peptide

Nakamura, M., Fujiwara, K. & Doi, N., 2022 12月, In: Journal of Nanobiotechnology. 20, 1, 458.

研究成果: Article › 査読

Open Access

Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells

Takada, S., Yoshinaga, N., Doi, N. & Fujiwara, K., 2022 6月, In: Science Advances. 8, 23, eabm8460.

研究成果: Article › 査読

2 被引用数 (Scopus)

2021

Activation of a diluted E. coli cell-free transcription-translation system within liposomes by hypertonic concentration

Matsui, Y., Akui, T., Doi, N. & Fujiwara, K., 2021 12月 17, In: STAR Protocols. 2, 4, 101003.

研究成果: Article › 査読

Open Access

1 被引用数 (Scopus)

A relationship between ntp and cell extract concentration for cell-free protein expression

Takahashi, K., Sato, G., Doi, N. & Fujiwara, K., 2021 3月, In: Life. 11, 3, 237.

研究成果: Article › 査読

Open Access

4 被引用数 (Scopus)

Switching ON of Transcription-Translation System Using GUV Fusion by Co-supplementation of Calcium with Long-Chain Polyethylene Glycol

Uwaguchi, Y., Fujiwara, K. & Doi, N., 2021 7月 1, In: ChemBioChem. 22, 13, p. 2319-2324 6 p.

研究成果: Article › 査読

System concentration shift as a regulator of transcription-translation system within liposomes

Akui, T., Fujiwara, K., Sato, G., Takinoue, M., Nomura, S. I. M. & Doi, N., 2021 8月 20, In: iScience. 24, 8, 102859.

研究成果: Article › 査読

Open Access

4 被引用数 (Scopus)

Transcription-translation of theEscherichia coligenome within artificial cells

Deyama, T., Matsui, Y., Chadani, Y., Sekine, Y., Doi, N. & Fujiwara, K., 2021 10月 14, In: Chemical Communications. 57, 80, p. 10367-10370 4 p.

研究成果: Article › 査読

2020

Conformational equilibrium of MinE regulates the allowable concentration ranges of a protein wave for cell division

Kohyama, S., Fujiwara, K., Yoshinaga, N. & Doi, N., 2020 6月 14, In: Nanoscale. 12, 22, p. 11960-11970 11 p.

研究成果: Article › 査読

9 被引用数 (Scopus)

Microfluidic screening system based on boron-doped diamond electrodes and dielectrophoretic sorting for directed evolution of NAD(P)-dependent oxidoreductases

Goto, H., Kanai, Y., Yotsui, A., Shimokihara, S., Shitara, S., Oyobiki, R., Fujiwara, K., Watanabe, T., Einaga, Y., Matsumoto, Y., Miki, N. & Doi, N., 2020 2月 21, In: Lab on a Chip. 20, 4, p. 852-861 10 p.

研究成果: Article › 査読

22 被引用数 (Scopus)

2019

Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells

Kohyama, S., Yoshinaga, N., Yanagisawa, M., Fujiwara, K. & Doi, N., 2019 7月, In: eLife. 8, e44591.

研究成果: Article › 査読

Open Access

27 被引用数 (Scopus)

Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system

Yoshida, A., Kohyama, S., Fujiwara, K., Nishikawa, S. & Doi, N., 2019, In: Chemical Science. 10, 48, p. 11064-11072 9 p.

研究成果: Article › 査読

Open Access

19 被引用数 (Scopus)

2018

A dual system using compartmentalized partnered replication for selection of arsenic-responsive transcriptional regulator

Aye, S. L., Fujiwara, K. & Doi, N., 2018 11月 1, In: Journal of biochemistry. 164, 5, p. 341-348 8 p.

研究成果: Article › 査読

2 被引用数 (Scopus)

Artificial Cell Fermentation as a Platform for Highly Efficient Cascade Conversion

Fujiwara, K., Adachi, T. & Doi, N., 2018 2月 16, In: ACS Synthetic Biology. 7, 2, p. 363-370 8 p.

研究成果: Article › 査読

14 被引用数 (Scopus)

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication

Aye, S. L., Fujiwara, K., Ueki, A. & Doi, N., 2018 5月 5, In: Biochemical and Biophysical Research Communications. 499, 2, p. 170-176 7 p.

研究成果: Article › 査読

9 被引用数 (Scopus)

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Sakai, A., Murayama, Y., Fujiwara, K., Fujisawa, T., Sasaki, S., Kidoaki, S. & Yanagisawa, M., 2018 4月 25, In: ACS Central Science. 4, 4, p. 477-483 7 p.

研究成果: Article › 査読

Open Access

22 被引用数 (Scopus)

2017

DNA cytoskeleton for stabilizing artificial cells

Kurokawa, C., Fujiwara, K., Morita, M., Kawamata, I., Kawagishi, Y., Sakai, A., Murayama, Y., Nomura, S. I. M., Murata, S., Takinoue, M. & Yanagisawa, M., 2017 7月 11, In: Proceedings of the National Academy of Sciences of the United States of America. 114, 28, p. 7228-7233 6 p.

研究成果: Article › 査読

Open Access

80 被引用数 (Scopus)

Human-derived fusogenic peptides for the intracellular delivery of proteins

Sudo, K., Niikura, K., Iwaki, K., Kohyama, S., Fujiwara, K. & Doi, N., 2017 6月 10, In: Journal of Controlled Release. 255, p. 1-11 11 p.

研究成果: Article › 査読

27 被引用数 (Scopus)

In vitro transcription-Translation using bacterial genome as a template to reconstitute intracellular profile

Fujiwara, K., Sawamura, T., Niwa, T., Deyama, T., Nomura, S. I. M., Taguchi, H. & Doi, N., 2017 11月 2, In: Nucleic acids research. 45, 19, p. 11449-11458 10 p.

研究成果: Article › 査読

10 被引用数 (Scopus)

Liposomal internal viscosity affects the fate of membrane deformation induced by hypertonic treatment

Fujiwara, K. & Yanagisawa, M., 2017, In: Soft matter. 13, 48, p. 9192-9198 7 p.

研究成果: Article › 査読

5 被引用数 (Scopus)

Universal glass-forming behavior of in vitro and living cytoplasm

Nishizawa, K., Fujiwara, K., Ikenaga, M., Nakajo, N., Yanagisawa, M. & Mizuno, D., 2017 12月 1, In: Scientific reports. 7, 1, 15143.

研究成果: Article › 査読

Open Access

35 被引用数 (Scopus)

2016

Biochemical preparation of cell extract for cell-free protein synthesis without physical disruption

Fujiwara, K. & Doi, N., 2016 4月, In: PloS one. 11, 4, e0154614.

研究成果: Article › 査読

35 被引用数 (Scopus)

Identification of novel in vivo obligate GroEL/ES substrates based on data from a cell-free proteomics approach

Niwa, T., Fujiwara, K. & Taguchi, H., 2016 1月 1, In: FEBS Letters. 590, 2, p. 251-257 7 p.

研究成果: Article › 査読

15 被引用数 (Scopus)

In vitro selection of bispecific diabody fragments using covalent bicistronic DNA display

Nakayama, M., Komiya, S., Fujiwara, K., Horisawa, K. & Doi, N., 2016 9月 16, In: Biochemical and Biophysical Research Communications. 478, 2, p. 606-611 6 p.

研究成果: Article › 査読

2 被引用数 (Scopus)

PURE mRNA display for in vitro selection of single-chain antibodies

Nagumo, Y., Fujiwara, K., Horisawa, K., Yanagawa, H. & Doi, N., 2016 5月 1, In: Journal of biochemistry. 159, 5, p. 519-526 8 p.

研究成果: Article › 査読

15 被引用数 (Scopus)

2015

Droplet-Shooting and Size-Filtration (DSSF) Method for Synthesis of Cell-Sized Liposomes with Controlled Lipid Compositions

Morita, M., Onoe, H., Yanagisawa, M., Ito, H., Ichikawa, M., Fujiwara, K., Saito, H. & Takinoue, M., 2015 9月 1, In: ChemBioChem. 16, 14, p. 2029-2035 7 p.

研究成果: Article › 査読

34 被引用数 (Scopus)

Generation of monodisperse cell-sized microdroplets using a centrifuge-based axisymmetric co-flowing microfluidic device

Yamashita, H., Morita, M., Sugiura, H., Fujiwara, K., Onoe, H. & Takinoue, M., 2015 4月 1, In: Journal of Bioscience and Bioengineering. 119, 4, p. 492-495 4 p.

研究成果: Article › 査読

19 被引用数 (Scopus)

Peptide sequences converting polyglutamine into a prion in yeast

Odani, W., Urata, K., Okuda, M., Okuma, S., Koyama, H., Pack, C. G., Fujiwara, K., Nojima, T., Kinjo, M., Kawai-Noma, S. & Taguchi, H., 2015 2月, In: FEBS Journal. 282, 3, p. 477-490 14 p.

研究成果: Article › 査読

Open Access

2014

Conversion of a chaperonin GroEL-independent protein into an obligate substrate

Ishimoto, T., Fujiwara, K., Niwa, T. & Taguchi, H., 2014 11月 14, In: Journal of Biological Chemistry. 289, 46, p. 32073-32080 8 p.

研究成果: Article › 査読

10 被引用数 (Scopus)

Generation of giant unilamellar liposomes containing biomacromolecules at physiological intracellular concentrations using hypertonic conditions

Fujiwara, K. & Yanagisawa, M., 2014 12月 19, In: ACS Synthetic Biology. 3, 12, p. 870-874 5 p.

研究成果: Article › 査読

31 被引用数 (Scopus)

Multiple patterns of polymer gels in microspheres due to the interplay among phase separation, wetting, and gelation

Yanagisawa, M., Nigorikawa, S., Sakaue, T., Fujiwara, K. & Tokita, M., 2014 11月 11, In: Proceedings of the National Academy of Sciences of the United States of America. 111, 45, p. 15894-15899 6 p.

研究成果: Article › 査読

29 被引用数 (Scopus)

2013

A bacterial salt sensor created by multiplying phenotypes of GroE-depleted Escherichia coli

Fujiwara, K., Aoi, K. B. & Nomura, S. I. M., 2013 11月 7, In: Analytical Methods. 5, 21, p. 5918-5922 5 p.

研究成果: Article › 査読

3 被引用数 (Scopus)

Condensation of an Additive-Free Cell Extract to Mimic the Conditions of Live Cells

Fujiwara, K. & Nomura, S. I. M., 2013 1月 17, In: PloS one. 8, 1, e54155.

研究成果: Article › 査読

27 被引用数 (Scopus)

Cooperative working of bacterial chromosome replication proteins generated by a reconstituted protein expression system

Fujiwara, K., Katayama, T. & Nomura, S. I. M., 2013 8月, In: Nucleic acids research. 41, 14, p. 7176-7183 8 p.

研究成果: Article › 査読

40 被引用数 (Scopus)

Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual f-bar domain proteins

Tanaka-Takiguchi, Y., Itoh, T., Tsujita, K., Yamada, S., Yanagisawa, M., Fujiwara, K., Yamamoto, A., Ichikawa, M. & Takiguchi, K., 2013 1月 8, In: Langmuir. 29, 1, p. 328-336 9 p.

研究成果: Article › 査読