α- and β-cell function in obese Zucker (fa/fa) rats: A study with the isolated perfused pancreas

Hiroshi Hirose, H. Maruyama, K. Kido, K. Ito, K. Koyama, Y. Tashiro, T. Saruta

研究成果: Article

9 引用 (Scopus)

抄録

The effects of various stimuli, including changes in glucose concentration, arginine, tyramine and noradrenaline, on insulin and glucagon secretion were investigated using isolated perfused pancreata of obese and lean male Zucker rats at 12 months of age. In Zucker fatty rats, the insulin secretion rate was significantly (P < 0.01) higher than that of lean rats at all glucose concentrations tested (8.3, 16.7 and 1.4 mmol/l). However, the integrated insulin secretory response to raising the glucose concentration from 8.3 to 16.7 mmol/l was almost absent in these rats. The glucagon secretion rates were significantly lower at 8.3 and 1.4 mmol/l glucose (P < 0.001 for both), and in responses to 10 μg/ml tyramine and 0.1 μmol/l noradrenaline (P < 0.05 for both), in Zucker fatty rats. Integrated insulin and glucagon responses to 10 mmol/l arginine were identical in the two groups. Histopathological and immunochemical studies revealed hyperplasia of β-cells and scattered α-cells in the enlarged islets of Zucker fatty rats. These results suggest that, in Zucker fatty rats, the decreased glucagon secretion in the isolated perfused pancreas is attributable to changes in the environment of α-cells and/or the inhibitory effects of hypersecreted insulin.

元の言語English
ページ(範囲)311-316
ページ数6
ジャーナルClinical Science
86
発行部数3
出版物ステータスPublished - 1994

Fingerprint

Zucker Rats
Pancreas
Glucagon
Insulin
Glucose
Tyramine
Arginine
Norepinephrine
Hyperplasia

ASJC Scopus subject areas

  • Medicine(all)

これを引用

Hirose, H., Maruyama, H., Kido, K., Ito, K., Koyama, K., Tashiro, Y., & Saruta, T. (1994). α- and β-cell function in obese Zucker (fa/fa) rats: A study with the isolated perfused pancreas. Clinical Science, 86(3), 311-316.

α- and β-cell function in obese Zucker (fa/fa) rats : A study with the isolated perfused pancreas. / Hirose, Hiroshi; Maruyama, H.; Kido, K.; Ito, K.; Koyama, K.; Tashiro, Y.; Saruta, T.

:: Clinical Science, 巻 86, 番号 3, 1994, p. 311-316.

研究成果: Article

Hirose, H, Maruyama, H, Kido, K, Ito, K, Koyama, K, Tashiro, Y & Saruta, T 1994, 'α- and β-cell function in obese Zucker (fa/fa) rats: A study with the isolated perfused pancreas', Clinical Science, 巻. 86, 番号 3, pp. 311-316.
Hirose H, Maruyama H, Kido K, Ito K, Koyama K, Tashiro Y その他. α- and β-cell function in obese Zucker (fa/fa) rats: A study with the isolated perfused pancreas. Clinical Science. 1994;86(3):311-316.
Hirose, Hiroshi ; Maruyama, H. ; Kido, K. ; Ito, K. ; Koyama, K. ; Tashiro, Y. ; Saruta, T. / α- and β-cell function in obese Zucker (fa/fa) rats : A study with the isolated perfused pancreas. :: Clinical Science. 1994 ; 巻 86, 番号 3. pp. 311-316.
@article{99240df2a5154fa1ab5101f94357a119,
title = "α- and β-cell function in obese Zucker (fa/fa) rats: A study with the isolated perfused pancreas",
abstract = "The effects of various stimuli, including changes in glucose concentration, arginine, tyramine and noradrenaline, on insulin and glucagon secretion were investigated using isolated perfused pancreata of obese and lean male Zucker rats at 12 months of age. In Zucker fatty rats, the insulin secretion rate was significantly (P < 0.01) higher than that of lean rats at all glucose concentrations tested (8.3, 16.7 and 1.4 mmol/l). However, the integrated insulin secretory response to raising the glucose concentration from 8.3 to 16.7 mmol/l was almost absent in these rats. The glucagon secretion rates were significantly lower at 8.3 and 1.4 mmol/l glucose (P < 0.001 for both), and in responses to 10 μg/ml tyramine and 0.1 μmol/l noradrenaline (P < 0.05 for both), in Zucker fatty rats. Integrated insulin and glucagon responses to 10 mmol/l arginine were identical in the two groups. Histopathological and immunochemical studies revealed hyperplasia of β-cells and scattered α-cells in the enlarged islets of Zucker fatty rats. These results suggest that, in Zucker fatty rats, the decreased glucagon secretion in the isolated perfused pancreas is attributable to changes in the environment of α-cells and/or the inhibitory effects of hypersecreted insulin.",
keywords = "glucagon, insulin, non-insulin-dependent diabetes mellitus, Zucker fatty rats",
author = "Hiroshi Hirose and H. Maruyama and K. Kido and K. Ito and K. Koyama and Y. Tashiro and T. Saruta",
year = "1994",
language = "English",
volume = "86",
pages = "311--316",
journal = "Clinical Science",
issn = "0143-5221",
publisher = "Portland Press Ltd.",
number = "3",

}

TY - JOUR

T1 - α- and β-cell function in obese Zucker (fa/fa) rats

T2 - A study with the isolated perfused pancreas

AU - Hirose, Hiroshi

AU - Maruyama, H.

AU - Kido, K.

AU - Ito, K.

AU - Koyama, K.

AU - Tashiro, Y.

AU - Saruta, T.

PY - 1994

Y1 - 1994

N2 - The effects of various stimuli, including changes in glucose concentration, arginine, tyramine and noradrenaline, on insulin and glucagon secretion were investigated using isolated perfused pancreata of obese and lean male Zucker rats at 12 months of age. In Zucker fatty rats, the insulin secretion rate was significantly (P < 0.01) higher than that of lean rats at all glucose concentrations tested (8.3, 16.7 and 1.4 mmol/l). However, the integrated insulin secretory response to raising the glucose concentration from 8.3 to 16.7 mmol/l was almost absent in these rats. The glucagon secretion rates were significantly lower at 8.3 and 1.4 mmol/l glucose (P < 0.001 for both), and in responses to 10 μg/ml tyramine and 0.1 μmol/l noradrenaline (P < 0.05 for both), in Zucker fatty rats. Integrated insulin and glucagon responses to 10 mmol/l arginine were identical in the two groups. Histopathological and immunochemical studies revealed hyperplasia of β-cells and scattered α-cells in the enlarged islets of Zucker fatty rats. These results suggest that, in Zucker fatty rats, the decreased glucagon secretion in the isolated perfused pancreas is attributable to changes in the environment of α-cells and/or the inhibitory effects of hypersecreted insulin.

AB - The effects of various stimuli, including changes in glucose concentration, arginine, tyramine and noradrenaline, on insulin and glucagon secretion were investigated using isolated perfused pancreata of obese and lean male Zucker rats at 12 months of age. In Zucker fatty rats, the insulin secretion rate was significantly (P < 0.01) higher than that of lean rats at all glucose concentrations tested (8.3, 16.7 and 1.4 mmol/l). However, the integrated insulin secretory response to raising the glucose concentration from 8.3 to 16.7 mmol/l was almost absent in these rats. The glucagon secretion rates were significantly lower at 8.3 and 1.4 mmol/l glucose (P < 0.001 for both), and in responses to 10 μg/ml tyramine and 0.1 μmol/l noradrenaline (P < 0.05 for both), in Zucker fatty rats. Integrated insulin and glucagon responses to 10 mmol/l arginine were identical in the two groups. Histopathological and immunochemical studies revealed hyperplasia of β-cells and scattered α-cells in the enlarged islets of Zucker fatty rats. These results suggest that, in Zucker fatty rats, the decreased glucagon secretion in the isolated perfused pancreas is attributable to changes in the environment of α-cells and/or the inhibitory effects of hypersecreted insulin.

KW - glucagon

KW - insulin

KW - non-insulin-dependent diabetes mellitus

KW - Zucker fatty rats

UR - http://www.scopus.com/inward/record.url?scp=0028350685&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028350685&partnerID=8YFLogxK

M3 - Article

C2 - 8156742

AN - SCOPUS:0028350685

VL - 86

SP - 311

EP - 316

JO - Clinical Science

JF - Clinical Science

SN - 0143-5221

IS - 3

ER -