16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique

Michiei Oto, Wataru Suda, Hirofumi Shinoyama

研究成果: Article

6 引用 (Scopus)

抄録

We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.

元の言語English
ページ(範囲)482-484
ページ数3
ジャーナルJournal of Bioscience and Bioengineering
102
発行部数5
DOI
出版物ステータスPublished - 2006 11
外部発表Yes

Fingerprint

Single-Stranded Conformational Polymorphism
Polymorphism
rRNA Genes
Amplification
Conformations
Bacteria
Genes
Genome
Polymerase chain reaction
DNA
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

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AU - Suda, Wataru

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AB - We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.

KW - 16S rRNA gene

KW - nonculturable bacteria

KW - polymerase chain reaction (PCR)

KW - single-strand conformation polymorphism (SSCP)

KW - whole-genome amplification (WGA)

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