16S rRNA gene-based analysis of microbial community by whole-genome amplification and minigel-single-strand conformation polymorphism technique

Michiei Oto, Wataru Suda, Hirofumi Shinoyama

研究成果: Article査読

6 被引用数 (Scopus)

抄録

We have developed an analytical technique for the 16S rRNA gene that comprises whole-genome amplification and the polymerase chain reaction (PCR)-minigel-single-strand conformation polymorphism technique (WGA-SSCP). Under optimal conditions, SSCP bands could be detected when genomic DNA from bacteria of interest comprised 0.5% or more of the specimen. This method will be effective for the identification of nonculturable bacteria in a microbial community.

本文言語English
ページ(範囲)482-484
ページ数3
ジャーナルJournal of Bioscience and Bioengineering
102
5
DOI
出版ステータスPublished - 2006 11

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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