2D DIGE proteomic analysis highlights delayed postnatal repression of α-fetoprotein expression in homocystinuria model mice

Shotaro Kamata, Noriyuki Akahoshi, Isao Ishii

研究成果: Article査読

3 被引用数 (Scopus)

抄録

Cystathionine β-synthase-deficient (Cbs-/-) mice, an animal model for homocystinuria, exhibit hepatic steatosis and juvenile semilethality via as yet unknown mechanisms. The plasma protein profile of Cbs-/- mice was investigated by proteomic analysis using two-dimensional difference gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight/mass spectrometry. We found hyperaccumulation of α-fetoprotein (AFP) and downregulation of most other plasma proteins. AFP was highly expressed in fetal liver, but its expression declined dramatically via transcriptional repression after birth in both wild-type and Cbs-/- mice. However, the repression was delayed in Cbs-/- mice, causing high postnatal AFP levels, which may relate to transcriptional repression of most plasma proteins originating from liver and the observed hepatic dysfunction.

本文言語English
ページ(範囲)535-541
ページ数7
ジャーナルFEBS Open Bio
5
DOI
出版ステータスPublished - 2015

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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