3′-Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA-binding protein

Kenichi Horisawa, Takao Imai, Hideyuki Okano, Hiroshi Yanagawa

研究成果: Article査読

29 被引用数 (Scopus)

抄録

Musashi1 (Msi1) is an RNA-binding protein that is highly expressed in neural stem cells, and is considered to be a stemness factor. A known function of Msi1 is translational repression of specifically bound mRNAs. Although the basic mechanism and some target RNAs have been reported, further survey of interactors is necessary to understand the integrated function of Msi1. By screening using an mRNA display technique, we found that doublecortin (dcx) mRNA is a specific binding target of Msi1 in vitro. We confirmed that Msil repressed translation of a luciferase reporter gene linked to the selected 3′-untranslated region fragment of dcx in Neuro2A cells.

本文言語English
ページ(範囲)2429-2434
ページ数6
ジャーナルFEBS Letters
583
14
DOI
出版ステータスPublished - 2009 7 21

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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