3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells

A. Y. Hsiao, T. Okitsu, Hiroaki Onoe, M. Kiyosawa, H. Teramae, S. Iwanaga, S. Miura, T. Kazama, T. Matsumoto, S. Takeuchi

研究成果: Conference contribution

抄録

Here we show that 3D fiber-shaped culture system enhances the differentiation of multipotent DFAT (dedifferentiated fat) cells into smooth muscle-like cells. DFAT cells cultured as 3D cell fiber constructs were induced to differentiate into smooth muscle cells. We found expression of smooth muscle lineage specific markers ASMA ( α-smooth muscle actin) and calponin in the differentiated DFAT cell fibers. The expression of calponin as marker of more mature smooth muscle cells has not been achieved in 2D cultures before. These findings suggest that 3D fiber microenvironment is able to promote more efficient differentiation into smooth muscle-like cells compared to 2D culture.

元の言語English
ホスト出版物のタイトル17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
出版者Chemical and Biological Microsystems Society
ページ410-412
ページ数3
1
ISBN(印刷物)9781632666246
出版物ステータスPublished - 2013
外部発表Yes
イベント17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
継続期間: 2013 10 272013 10 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Germany
Freiburg
期間13/10/2713/10/31

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Oils and fats
Muscle
Fats
Fibers
Cells
Cell culture
Actins

ASJC Scopus subject areas

  • Bioengineering

これを引用

Hsiao, A. Y., Okitsu, T., Onoe, H., Kiyosawa, M., Teramae, H., Iwanaga, S., ... Takeuchi, S. (2013). 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (巻 1, pp. 410-412). Chemical and Biological Microsystems Society.

3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. / Hsiao, A. Y.; Okitsu, T.; Onoe, Hiroaki; Kiyosawa, M.; Teramae, H.; Iwanaga, S.; Miura, S.; Kazama, T.; Matsumoto, T.; Takeuchi, S.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1 Chemical and Biological Microsystems Society, 2013. p. 410-412.

研究成果: Conference contribution

Hsiao, AY, Okitsu, T, Onoe, H, Kiyosawa, M, Teramae, H, Iwanaga, S, Miura, S, Kazama, T, Matsumoto, T & Takeuchi, S 2013, 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻. 1, Chemical and Biological Microsystems Society, pp. 410-412, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 13/10/27.
Hsiao AY, Okitsu T, Onoe H, Kiyosawa M, Teramae H, Iwanaga S その他. 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. : 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1. Chemical and Biological Microsystems Society. 2013. p. 410-412
Hsiao, A. Y. ; Okitsu, T. ; Onoe, Hiroaki ; Kiyosawa, M. ; Teramae, H. ; Iwanaga, S. ; Miura, S. ; Kazama, T. ; Matsumoto, T. ; Takeuchi, S. / 3D fiber-shaped culture system promotes differentiation of multi potent DFAT cells into smooth muscle-like cells. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 巻 1 Chemical and Biological Microsystems Society, 2013. pp. 410-412
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AU - Teramae, H.

AU - Iwanaga, S.

AU - Miura, S.

AU - Kazama, T.

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