3D visualization of epidermal langerhans cells

Akiharu Kubo, Keisuke Nagao, Masayuki Amagai

研究成果: Chapter

9 被引用数 (Scopus)

抄録

Langerhans cells (LCs) are intraepidermal dendritic cells that extend their dendrites between keratinocytes to form a dense network that covers the entire body. The mammalian epidermis has two diffusion barriers, the stratum corneum and tight junctions (TJs). In their resting state, LCs sit underneath these two barriers. Once activated, LCs elongate their dendrites to dock with, or penetrate through, TJs, which allows them to survey the environment between the two barriers. Here, we describe a method to visualize this dynamic interaction of TJs and LCs in 3D by using mouse ear epidermal sheets.

本文言語English
ホスト出版物のタイトルMolecular Dermatology
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ119-127
ページ数9
ISBN(印刷版)9781627032261
DOI
出版ステータスPublished - 2013

出版物シリーズ

名前Methods in Molecular Biology
961
ISSN(印刷版)1064-3745

ASJC Scopus subject areas

  • 分子生物学
  • 遺伝学

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