A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA

Jun Katahira, Haruhiko Siomi, Toshimasa Ishizaki, Tomoe Umemoto, Yuetsu Tanaka, Hisatoshi Shida

研究成果: Article

4 引用 (Scopus)

抄録

We examined the cellular protein(s) which can associate with Rex protein of human T cell leukemia virus type I (HTLV-I), using Rex-maltose binding protein (MBP) fusion protein. Immunoprecipitation of RexMBP with anti-MBP antibody revealed that a 24 kD protein (p24) associated with RexMBP only in the presence of Rex-responsive mRNA, The fact that p24 was present in both the nucleus and the cytoplasm is consistent with a role of Rex in the nucleo-cytoplasmic transport of viral mRNAs. P24 did not interact with nonfunctional Rex mutant proteins even if they had RNA binding activity in vitro. These results suggest the possible involvement of p24 in the Rex function through a complex formation with Rex on Rex-responsive mRNA.

元の言語English
ページ(範囲)3535-3544
ページ数10
ジャーナルOncogene
9
発行部数12
出版物ステータスPublished - 1994 12
外部発表Yes

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Maltose-Binding Proteins
Messenger RNA
rex Gene Products
Proteins
Cell Nucleus Active Transport
Mutant Proteins
Immunoprecipitation
Cytoplasm
RNA
Antibodies
Human T-lymphotropic virus 1 rex Protein
In Vitro Techniques

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

これを引用

Katahira, J., Siomi, H., Ishizaki, T., Umemoto, T., Tanaka, Y., & Shida, H. (1994). A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA. Oncogene, 9(12), 3535-3544.

A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA. / Katahira, Jun; Siomi, Haruhiko; Ishizaki, Toshimasa; Umemoto, Tomoe; Tanaka, Yuetsu; Shida, Hisatoshi.

:: Oncogene, 巻 9, 番号 12, 12.1994, p. 3535-3544.

研究成果: Article

Katahira, J, Siomi, H, Ishizaki, T, Umemoto, T, Tanaka, Y & Shida, H 1994, 'A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA', Oncogene, 巻. 9, 番号 12, pp. 3535-3544.
Katahira J, Siomi H, Ishizaki T, Umemoto T, Tanaka Y, Shida H. A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA. Oncogene. 1994 12;9(12):3535-3544.
Katahira, Jun ; Siomi, Haruhiko ; Ishizaki, Toshimasa ; Umemoto, Tomoe ; Tanaka, Yuetsu ; Shida, Hisatoshi. / A cellular protein which is coprecipitated with HTLV-I rex protein in the presence of the target mRNA. :: Oncogene. 1994 ; 巻 9, 番号 12. pp. 3535-3544.
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