A continuous-flow microbial microreactor using microbes immobilized into a microporous carrier by dielectrophoresis

T. Kano, T. Inaba, Y. Gu, N. Miki

研究成果: Conference contribution

1 被引用数 (Scopus)

抄録

In this paper we demonstrate a continuous-flow microbial microreactor that immobilizes microbes into microporous carrier. First, bacteria are trapped into 3, 5, 10, 20-μm-diameter pores using positive dielectrophoresis (DEP). After non-immobilized bacteria are flushed, the continuous-flow microreactor generates reaction products when culture media including reactive substrate are supplied. Continuous-flow type reactors facilitate collecting and evaluating reaction products. We used the developed microreactor to characterize microbes belonging to Corynebacterium group by measuring generated lactic acid when glucose was supplied. The amount of lactic acid produced by a single bacterium was deduced and we found Corynebacterium variabile to be most productive among three tested members. The microbial reactor proposed herein is readily applicable to an efficient microbial screening platform.

本文言語English
ホスト出版物のタイトル2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
ページ745-748
ページ数4
DOI
出版ステータスPublished - 2012
イベント2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
継続期間: 2012 1 292012 2 2

出版物シリーズ

名前Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN(印刷版)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
国/地域France
CityParis
Period12/1/2912/2/2

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 凝縮系物理学
  • 機械工学
  • 電子工学および電気工学

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