A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation

Lia Supino-Rosin, Akihiko Yoshimura, Hanna Altaratz, Drorit Neumann

研究成果: Article

28 引用 (Scopus)

抄録

The erythropoietin receptor (EPO-R) is the cellular target for erythropoietin (EPO), the primary hormone that mediates the proliferation of immature erythroblasts and their differentiation into mature erythrocytes. Unusual features of the EPO-R are its short half-life (t( 1/2 ) 1-2 h), its degradation via multiple pathways and the fact that less than 1% of total cellular EPO-R molecules are found on the cell surface. The contribution of EPO-R structural determinants to the regulation of its intracellular metabolism is still unclear. The epidermal growth factor receptor (EGF-R), unlike the EPO-R, is efficiently transported to the cell surface and displays a much longer metabolic half-life. To determine which EPO-R cytosolic domains are involved in intracellular degradation, we studied chimeric receptor molecules constructed of EGF-R extracellular and transmembrane parts, linked to the full length or truncated cytosolic part of the EPO-R. The chimeras were expressed in transiently transfected COS 7 cells and stably expressed in Ba/F3 cells. Our experiments indicate that the cytosolic part of the EPO-R contains determinants that mark it for rapid degradation, in association with the endoplasmic reticulum (ER). This degradation was insensitive to brefeldin A and was inhibited by specific proteasomal inhibitors. A truncated EGF- R/EPO-R chimera containing only 50 amino acids of the EPO-R membrane-proximal cytosolic part was also rapidly degraded suggesting that these 50 amino acids are involved in receptor degradation.

元の言語English
ページ(範囲)410-419
ページ数10
ジャーナルEuropean Journal of Biochemistry
263
発行部数2
DOI
出版物ステータスPublished - 1999 7 15
外部発表Yes

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Endoplasmic Reticulum-Associated Degradation
Erythropoietin Receptors
Degradation
Erythropoietin
Epidermal Growth Factor Receptor
Half-Life
Amino Acid Receptors
Brefeldin A
Amino Acids
Erythroblasts
Molecules
COS Cells
Epidermal Growth Factor
Metabolism
Endoplasmic Reticulum
Erythrocytes
Association reactions
Hormones
Membranes

ASJC Scopus subject areas

  • Biochemistry

これを引用

A cytosolic domain of the erythropoietin receptor contributes to endoplasmic reticulum-associated degradation. / Supino-Rosin, Lia; Yoshimura, Akihiko; Altaratz, Hanna; Neumann, Drorit.

:: European Journal of Biochemistry, 巻 263, 番号 2, 15.07.1999, p. 410-419.

研究成果: Article

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