A fairy chemical suppresses retinal angiogenesis as a HIF inhibitor

Deokho Lee, Yukihiro Miwa, Jing Wu, Chiho Shoda, Heonuk Jeong, Hirokazu Kawagishi, Kazuo Tsubota, Toshihide Kurihara

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Neovascular retinal degeneration is a leading cause of blindness in advanced countries. Anti-vascular endothelial growth factor (VEGF) drugs have been used for neovascular retinal diseases; however, anti-VEGF drugs may cause the development of chorioretinal atrophy in chronic therapy as they affect the physiological amount of VEGF needed for retinal homeostasis. Hypoxia-inducible factor (HIF) is a transcription factor inducing VEGF expression under hypoxic and other stress conditions. Previously, we demonstrated that HIF was involved with pathological retinal angiogenesis in murine models of oxygen-induced retinopathy (OIR), and pharmacological HIF inhibition prevented retinal neovascularization by reducing an ectopic amount of VEGF. Along with this, we attempted to find novel effective HIF inhibitors. Compounds originally isolated from mushroom-forming fungi were screened for prospective HIF inhibitors utilizing cell lines of 3T3, ARPE-19 and 661W. A murine OIR model was used to examine the anti-angiogenic effects of the compounds. As a result, 2-azahypoxanthine (AHX) showed an inhibitory effect on HIF activation and suppressed Vegf mRNA upregulation under CoCl2-induced pseudo-hypoxic conditions. Oral administration of AHX significantly suppressed retinal neovascular tufts in the OIR model. These data suggest that AHX could be a promising anti-angiogenic agent in retinal neovascularization by inhibiting HIF activation.

本文言語English
論文番号1405
ページ(範囲)1-14
ページ数14
ジャーナルBiomolecules
10
10
DOI
出版ステータスPublished - 2020 10

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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