A finger sweat chloride test for the detection of a high-risk group of chronic pancreatitis

Satoru Naruse, Hiroshi Ishiguro, Yasufumi Suzuki, Kotoyo Fujiki, Shigeru B.H. Ko, Nobumasa Mizuno, Toshihiro Takemura, Akiko Yamamoto, Toshiyuki Yoshikawa, Chunxiang Jin, Ryujiro Suzuki, Motoji Kitagawa, Takao Tsuda, Takaharu Kondo, Tetsuo Hayakawa

研究成果: Article

14 引用 (Scopus)

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Objectives: Mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene are associated with chronic pancreatitis in Caucasians. We developed a simple method for measuring finger sweat chloride concentration to test whether CFTR dysfunction underlies chronic pancreatitis in Japan where cystic fibrosis (CF) is rare. Methods: We studied 25 patients with chronic (21 alcoholic and 4 idiopathic) pancreatitis and 25 healthy volunteers. Sweat chloride concentrations were measured by a finger sweat chloride test. We analyzed DNA for 20 common CFTR mutations in Europeans, 9 CF-causing mutations in Japanese, and 2 polymorphic loci, a poly-T tract and (TG) repeats, at intron 8. Results: Thirteen patients (52%) had sweat chloride levels >60 mmol/L, a level consistent with CF, while only 4 (16%) healthy subjects exceeded this level. The 29 CF mutations and the 5T allele were detected in neither the patients nor controls. The (TG) 12 allele was common in both the patients (58%) and controls (48%). The (TG) 12/12 genotype was common in alcoholic pancreatitis (29%) compared with the (TG) 11/11 (10%). Patients with the (TG) 12/12 genotype had significantly higher sweat chloride concentrations than the controls. Conclusion: CFTR dysfunction as evidenced by a finger sweat chloride test is present in about half of Japanese patients with chronic pancreatitis, suggesting that this test may be useful for detecting the high-risk group. A higher proportion of the (TG) 12 allele may be a genetic background for elevated sweat chloride concentrations in Japanese patients.

元の言語English
ページ(範囲)e80-e85
ジャーナルPancreas
28
発行部数3
DOI
出版物ステータスPublished - 2004 3

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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    Naruse, S., Ishiguro, H., Suzuki, Y., Fujiki, K., Ko, S. B. H., Mizuno, N., Takemura, T., Yamamoto, A., Yoshikawa, T., Jin, C., Suzuki, R., Kitagawa, M., Tsuda, T., Kondo, T., & Hayakawa, T. (2004). A finger sweat chloride test for the detection of a high-risk group of chronic pancreatitis. Pancreas, 28(3), e80-e85. https://doi.org/10.1097/00006676-200404000-00029